고정화 Pseudomonas aeruginosa를 이용한 질소제거에 관한 연구

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The aim of this research is to develop a new process of Nitrogen removal. Whole cells of Pseudomonas aeruginosa AE-1-3 were immobilized by entrapment with polyethlene glycol(PEG), and various factors on the removal of nitrogen from synthetic wastewater were investigated by batch and continuous reactors.
The removal rate of NO3-N increased with increasing of cell concentration in the immobilized beads. A higher removal rate of NO3-N were observed when the smaller bead size and more visual column of the immobilized beads were used. When glucose, was added as a carbon source, 50 mg/ℓ of NO3-N was completely removed in 9 hours. other carbon source, such as methanol was not utilized.
NO3-N was completely consumed at C/N of 5 and 10 and 20, but C/N 2.5 the removal efficiencies of NO3-N ranged from 76%. The removal efficiencies of NO3-N increased with increasing of temperature and the NO3-N removal efficiency of 96.8% could achieved at the low temperature(10℃) by the immobilized P. aeruginosa AE-1-3. The average removal efficiencies of NO3-N at the HRT of 24 and HRT of 12 was 97.4% and 98.8% respectively during continuous treatment by the immobilized P. aeruginosa AE-1-3 when NH4NO3 as a nitrogen source was used.
Alternative Title
A Study on the Removal of Nitrogen by Immobilized Pseudomonas aeruginosa
Alternative Author(s)
Gyoung Hun Park
조선대학교 공과대학
일반대학원 환경공학과
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Table Of Contents
목 차

List of Tables ⅲ
List of Figures ⅳ
Ⅰ. 서 론 1

Ⅱ. 이론적 고찰 4

1. 생물학적 탈질화 3
1) 탈질화 반응 3
2) 탈질화 영향인자 5
2. 미생물 고정화법 9
1) 고정화법의 정의 및 역사 9
2) 고정화법의 종류 및 특성 11
3) 포괄고정화법의 원리 15
4) 포괄고정화 재료 및 조건 16

Ⅲ. 실험방법 및 재료 17

1. 실험재료 17
1) 균주 17
2) 합성배지 17
2. 고정화 재료 및 방법 18
3. 실험장치 19
1) 회분식 실험장치 19
2) 연속식 실험장치 20
4. 실험방법 21
5. 분석방법 23
Ⅳ. 결과 및 고찰 24

1. 회분식 실험 24
1) 고정화 P. aeruginosa AE-1-3의 균체농도에 따른 NO3-N 제거 24
2) 고정화 P. aeruginosa AE-1-3의 고정화 비드크기에 따른 NO3-N의 제거 26
3) 고정화 P. aeruginosa AE-1-3의 충진율에 따른 NO3-N의 제거 28
4) 고정화 P. aeruginosa AE-1-3의 C/N비에 따른 NO3-N의 제거 30
5) 고정화 P. aeruginosa AE-1-3의 탄소원에 따른 NO3-N의 제거 32
6) 고정화 P. aeruginosa AE-1-3의 NO3-N의 농도에 따른 제거 34
7) 고정화 P. aeruginosa AE-1-3의 pH에 따른 NO3-N의 제거 36
8) 고정화 P. aeruginosa AE-1-3의 온도에 따른 NO3-N의 제거 38
2. 연속식 실험 40
1) 고정화 P. aeruginosa AE-1-3의 NH4-N, NO3-N의 연속 제거 40

Ⅴ. 결 론 42
박경훈. (2009). 고정화 Pseudomonas aeruginosa를 이용한 질소제거에 관한 연구.
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General Graduate School > 3. Theses(Master)
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