천일염된장 및 된장종균 Bacillus subtilis DJI의 암세포 성장 억제효과

Abstract

This study extracted anticancer Bacillus subtilis DJI from 4 types of strains separated from traditional fermented foods. The results of measuring the anticancer effect of B. subtilis DJI supernatant by cultivation phase according to the MTT assay method showed a high concentration-dependent cancer cell restraint effect for HT-29(human colon cancer cell) and AGS(human gastric adenocarcinoma cell), but, uniquely, showed a concentration-dependent cell growth effect for BJ(human foreskin normal cell). The anticancer effect of supernatant on cancer cells by cultivation phase was high in the order of the death phase, stationary phase and logarithmic phase, which confirmed that the closer to the death phase, the bigger anticancer effect was. The extraction of cell wall content from B. subtilis DJI showed a yield of 59%, and the measurement of the anticancer effect of B. subtilis DJI cell wall showed a high restraint rate for HT-29, but little cell toxicity for BJ. DJI doenjang was prepared by using B. subtilis DJI starter and the refined salt or solar salt with 12%(w/v) concentration. The prepared doenjangs were fermented and aged for 2 month and 16 month respectively. MTT assay was used to measure the growth-inhibitory effect of DJI doenjang extracts(water, methanol) on BJ, HT-29 and AGS. The anticancer effect increased in both refined salt-doenjang and solar salt-doenjang as fermentation period increased. In particular, in the case of water extracts, solar salt-doenjang showed much better anticancer effect than refined salt-doenjang. In addition, apoptosis were observed when the water extracts of the doenjangs were treated into AGS. In particular, it was observed that more apoptosis occured in solar salt-doenjang treats than refined salt-doenjang treats.