자운영 종자 아세톤 추출물의 피부 생리활성 효과와 피부미용에 미치는 영향

Abstract

This study aims to identify the skin bioactive effects of acetone extract from seed of Chinese milk vetch and its effect on skin care. For the purpose, we used acetone for skin bioactive test and made fluid and pack for which 0.2% an 0.5% of acetone was added for the skin care test, targeting 36 female adults. The results were as follows: Total content of polyphenol and flavonoid were 190.59 mg/g, and 63.03 mg/g, respectively. To check cytotoxicity in it, conducted a MTT assay and almost 100% of cell survival rate and cell proliferation were foun, which indicates that there was little cytotoxicity. As a result of testing DPPH radical scavenging effects, it was found that the acetone is a an anti-oxidant as it had high scavenging effects. The inhibitory activity of the elastase was 97.36% at a concentration of 250 ㎍/㎖, that of MMP-1 was 95.24% at a concentration of 500 ㎍/㎖, and that of tyrosinase was 94.41% at a concentration of 50 ㎎/㎖, which indicates that the elastase is effective for anti-aging and whitening of skin. The fluid where 0.2% and 0.5% acetone was added was stably liquidified and absorbed over the skin, which means good for moisture of skin, and the pack which was made from the fluid was evenly absorbed to skin and well removed after skin was dried. As a result of irritation test, fluid and pack made from seeds of milk vetch, had no irritation to skin at all. The fluid and pack were applied two experimental groups for eight weeks to measure facial conditions such as moisture, elasticity, coarseness, and pigment. skins of the two test groups showed better conditions after the use of fluid and pack (p<0.001), The test group who was applied 0.2% acetone showed better condition on the skin than the group who was applied 0.5% acetone.