오염된 결핵균 검체에서 PCR과GenoType MTBDRplus를 이용한신속 결핵균체검사법

Abstract

There are several methods to diagnose a tuberculosis patient, such as smear, PCR, tuberculosis culture, X～ray. For a proper treatment in the medical treatment, antimicrobial susceptibility test and rapid drug susceptibility testing wouldl be operated. Tuberculosis bacilli usually needs from 3～8 weeks of culture period because it synthesis delay RNA and needs 15～22 hours for generation. After a germ raises in culture, we put in operation antimicrobial susceptibility test for a proper treatment. It has some difficulties to give a proper prescription for a tuberculosis patient because antimicrobial susceptibility test requires 4 weeks. To supplement this, we are developing and practicing drug susceptibility testing which makes us know sensibility of RMP and INH that is representative tuber after 2 or 3 days. But this is possible just when more than 2 positive germs are detected and impossible with a small quantity of germ, we should practice rapid drug susceptibility testing with culture test. But if media is polluted by other germs except Mycobacterium tuberculosis, it's hard to interpret result about culture test and to practice antimicrobial susceptibility test and rapid drug susceptibility testing. Because we have to practice again smear, culture test after extracting specimen from the patient, time is consumed and proper patient treatment is postponed. To make up for these discomfort and quick patient treatment, rapid drug susceptibility testing is practiced by using GenoType MTBDRplus method. As a result, we gained sensibility 7 case and resistance 3 case other 1 case toward medicals from the total 11 test. By this, we gained conclusion that we can practice rapid drug susceptibility testing from the polluted specimen after eliminate a pollution source in culture and proved that we can practice rapid examination for a tuberculosis patient.