식나무(Aucuba japonica Thunb.)뿌리로부터 트리테르페노이드 화합물의 분리 및 구조규명

Abstract

Aucuba japonica Thunb (Cornaceae) is an evergreen shrub that is distributed throughout Korea (Ulleng, Jeju, Chonnam, Kyungnam) and Japan. Previous studies on the leaves of A. japonica, some iridoid compounds, such as aucubin, aucubigenin were reported. During our search for the phytochemical constituents of the methylene chloride soluble fraction of the roots of A. japonica, six triterpenoid compounds and one steroid compound, friedelin (1), 3α-hydroxy-2-friedelanone (2), β-sitosterol (3), canophyllol (4), oleanolic aldehyde acetate (5), ursolic acid (6), pachysandiol A (7) were isolated by the repeated silica gel, Sephadex LH-20 and MCI gel column chromatography. The chemical structures of compounds 1-7 were determined by the basis of physicochemical properties and spectroscopic methods such as 1D and 2D NMR. These compounds were isolated from this plant for the first time. For the isolated compounds, the inhibitory activity of IL-6 production in TNF-α stimulated MG-63 was examined. Among the isolated compounds, 3α-hydroxy-2-friedelanone (2) showed potent inhibitory effect on IL-6 production in TNF-α stimulated MG-63.