쇠고기 분쇄육의 감마선 및 전자선 조사에 의해 유도된 Hydrocarbon류 분석

Abstract

The purpose of the study was the possibility of identification by radiation-induced hydrocarbons analysis from irradiated ground beef. This experiment is comparison of vacuum and N2 gas packing methods and gamma ray and electron beam. When triglycerides are irradiated, hydrocarbons formed from each fatty acid. Radiation-induced hydrocarbons of ground beef was determined sequential procedure of lipid extraction by hexane, hydrocarbon isolation by florisil column chromatography and quantitative analysis by gas chromatography. As a result, radiation-induced hydrocarbons of ground beef was detected two types. First hydrocarbon type is one carbon less than parent fatty acid. Second hydrocarbon type is two carbon less than parent fatty acid and additional formed double bond at first carbon position. The major hydrocarbons in the irradiated ground beef was 8-heptadecene(C17:1) and 1,7-hexadecadiene(C16:2) from oleic acid. These hydrocarbons were high concentration but not detected in non-irradiated ground beef. Also, Radiation induced hydrocarbons are linear increase when the increase in the irradiation absorbed doses. And effects of gamma ray and electron beam radiation were similar at the same absorbed doses. But a higher level of radiation-induced hydrocarbons in gamma-rays has been confirmed than electron beam. Also, regression(R2) is gamma ray closer than electron beam to the 1. Because gamma ray is strong illuminate than electron beam. And effects of vacuum package and N2 gas package were similar at the same radiation absorbed doses. But a higher level of radiation-induced hydrocarbons in vacuum package has been confirmed than N2 gas package. Also, regression(R2) is vacuum package closer than N2 gas package to the 1. This is the meaning of investigation was well vacuum package than N2 gas package.