상피성 난소종양의 성장과 진행에서 p27, p21, p53, Ki-67 및 cyclin E 발현의 역할에 대한 면역학적 연구

Abstract

Objective : An ovarian surface epithelial tumor is a heterogeneous group of neoplasms with several different histologic types and various biological and molecular factors are important for its development and progression. Disturbances of the cell cycle regulatory proteins are key events underlying the development and/or progression of human malignance. The aim of this study was to further evaluate whether the expression of p27, p21, p53, Ki-67 and cyclin E is related to the malignancy of ovarian epithelial tumors and whether their expressions, alone or in combination, are associated with development and prognossion in epithelial ovarian carcinoma. Methods : Immunohistochemical analysis using anti p27, anti p21, anti-p53, anti-Ki-67 and anti-cyclin E antibodies was carried out for 93 cases consisting of benign, borderline and malignant ovarian epithelial (serous and mucinous) tumors. Results : The expression of p21 was increased in boderline serous tumor and serous cystadenocarcinoma in contrast to benign serous tumors. The expression of p21 was increased, benign mucinous tumors and borderline mucinous tumors in contrast to mucinous cystadenocarcinoma. The expression of p27 was reduced in mucinous cystadenocarcinomas in contrast to benign mucinous cystadenoma and borderline mucinous tumors. The expression of p53 was in borderline serous and mucinous tumors than benign and malignant tumor, whereas ovarian epithelial carcinoma showed high expression. The expression of Ki-67 was a gradually increased from benign to borderline to malignant ovarian epithelial tumors. The expression of cyclin E was a gradually increased from benign to borderline to malignant ovarian epithelial tumors. The expression of cyclin E, p21, p53 and Ki-67 correlated with type of tumor in the ovarian epithelial tumors. The expression of p53 and p21, p27, Ki-67 and cyclin E correlated with the type of ovarian epithelial tumors. Conclusions : This results suggest that loss of p27 expression and overexpression of p53, p21, Ki-67 and cyclin E were significantly associated with malignancy in ovarian epithelial tumors. P53, Ki-67 and cyclin E proteins may be valuable prognostic factors for epithelial ovarian carcinoma. Key words : tumor, p27, p21, p53, Ki-67, cyclin E, ovarian.