간흡충 감염 및 치료에 따른 간흡충 특이 항원의 면역조직화학적 부위별 특성 고찰

Abstract

To investigate the localization of Clonorchis sinensis antigens in adult worm of C. sinensis, according to infection and post-treatment, the immunohistochemical staining was adopted in the adult worm tissue of 27 weeks old C. sinensis. The immunohistochemical staining was used by the kit of Vector or by this laboratory method. The five rabbits infected with 150 or 450 C. sinensis metacercariae which were collected from the fresh-water fish, Pseudorasbora parva caught at the Nak-dong river. The infection(I) and post-treatment(T) sera were collected at I2 days(D), I12 weeks(W), I37W, T6 month(M), T12M, and T13M. The intensity of the immunohistochemical staining was determined by 5 steps in each organ. In infection, the intensity of staining of the intestine and intestinal contents were the most strongest (+++), that of the parenchyma, tegument, and eggs were the next strongest (++). The other organs such as testes, suckers, seminal receptacle, and vitelline glands, and ovary were positive(+) or weakly positive (±). The staining intensities in infection period were maintained from I2D to I37W. In post-treatment period, the intensities were slowly down-graded from T6M and converted negative at T12M or T13M. In the reaction with monoclonal antibodies, the parenchyma appeared positive with C. sinensis-specific monoclonal antibody. In the staining with human sera, the outer surface of the tegument showed positive with C. sinensis-positive patient serum. In this context, the cause of intestine and its contents showed to be the strongest staining was mightly mixed with specific and non-specific IgG reactions. Although in infection period, from early 2 days, the staining was positive, but in post-treatment period, at T13M, the staining was still positive. In contrast, the parenchyma, tegument, and eggs were observed to be the moderate strong in staining in infection period but converted one positive or negative in post-treatment period. Especially, the parenchyma was converted negative at T13M. Moreover, the parenchyma showed C. sinensis specificity with monoclonal antibody, indicating the parenchyma was specific in both infection and post-treatment. Therefore, C. sinensis-specific antigenicities were mainly derived from the intestine and intestinal contents, parenchyma, tegument, and eggs in infection period from early 2 days following the infection, however, in post-treatment period the reactions were down-graded at 12 or 13 months following the treatment, suggesting the parenchyma appeared positive reaction in the infection and turned to the negative conversion.

Key words : Clonorchis sinensis, Immunohistochemistry, Infection, Post-treatment, Intestine, Intestinal contents, Parenchyma, Tegument, Eggs