피부 각질세포의 과증식성 병변에서 Ki-67, p27, p53 단백 계열과 bcl-2의 발현.

Abstract

ABSTRACT

Expression of Ki-67, p27, p53 protein family and bcl-2 in keratinocytic proliferative lesions of the skin

Ryu, Reou-Sun Advisor: Prof. Suh, Chae-Hong, Ph.D. Department of Medicine, Graduate School, Chosun University

Background: Ki-67 is a nuclear antigen expressed in actively cycling cells but not the resting Go cells, and is frequently used as a marker for cell proliferation in tissue sections. P27 is a member of a family of CDK inhibitors that bind to cyclin/CDK complexes and arrest cell division. There is a considerable evidence that p27 plays an important role in multiple fundamental cellular proceses, including cell proliferation, cell differentiation and apoptosis. The p53 protein family is essential for the regulation of cell proliferation and its aberrant accumulation is usually seen in malignant tumors, but also occurs in squamous epithelium of inflammatory skin diseases characterized by hyperproliferation. P53, p63 and p73 are a tumor suppressors that likely play a role in the development of squamous cell carcinoma and possibly benign skin tumors. Several proteins of the bcl-2 gene family have been implicated in the regulation of programed cell death and cell proliferation. The keratinocytic hyperpoliferative skin lesions encompass wide of noncancerous, precancerous and cancerous. Methods; Immunohistochemical studies were performed on formalin-fixed, paraffin-embedded tissue sections. This study was compared the immunohistochemical findings using antibodies for Ki-67, p27, p53, p63, p73 and bcl-2 protein, in 15 cases of seborrheic keratosis, 11 cases of keratoacanthoma, 12 cases of actinic keratosis, 7 cases of Bowen's diseases and 11 cases of squamuos cell carcinomas (SCC). Results: Expression of Ki-67 was increased in actinic keratosis, Bowen's disease and SCC than in seborrhric keratosis and keratoacanthoma. The significantly higher p27 expression were observed in actinic keratosis, Bowen's disease and SCC than in seborrhric keratosis and keratoacanthoma. P53 & p73 expression were increased in actinic keratosis, Bowen's disease and SCC. P63 expression was increased in seborrheic keratosis, actinic keratosis, Bowen's disease and SCC. Expression of bcl-2 was slightly increased in seborrheic keratosis and actinic keratosis. There was a significant positive correlations among the expression of Ki-67, p27, p53, p63 and p73 in seborrheic keratosis, keratoacanthoma, actinic keratosis, Bowen's disease and SCC. Conclusion: These results demonstrate that increased expression of the Ki-67, p27 and p53 family protein (p53, p63 and p73) is a common finding in actinic keratosis, Bowen's disease and SCC of the skin. These findings suggest that Ki-67, p27, p53 family (p53, p63 p73) protein may play an important role of oncogenesis and ketatinocyte proliferation and differentiation in the skin neoplasms.