식중독균 오염 경로 추적을 위한 PFGE 활용 방안

Abstract

ABSTRACT

Application of pulsed-field gel electrophoresis(PFGE) for tracing the route of foodborne pathogens contamination

Choi, Soon Gon Advisor : Prof. Chang, Hae-choon, Ph. D. Department of Food and Drug Graduate School of Chosun University

To investigate foodborne disease we performed a prospective PFGE study and retrospective analysis of foodborne disease outbreaks in Korea. The prevention of foodborne disease requires a coordinated program of epidemiological study and rapid inspection involving tracing the route of foodborne pathogens contamination. Pulsed-field gel electrophoresis (PFGE) is a powerful tool for characterizing various foodborne pathogens at the DNA level, obtaining relevant information on genome size and constructing the physical and genetic map of the chromosome of bacteria that are poorly understood at the genetic level. PulseNet is a national network of public health and food regulatory established in the US to detect clusters of foodborne disease and respond quickly to foodborne outbreak investigations. In this active surveillance system detected by the network must be confirmed by epidemiological mean to determine if an outbreak is occurring. Presently, the preferred subtyping method of PulsedNet is pulsed-field gel electrophoresis (PFGE). And speeds outbreak investigations through focusing efforts on cases with indistinguishable PFGE patterns. Monitor for foodborne disease associated with a product and cut out foodborne illness. The subtyping of foodborne pathogens continuously monitors patterns submitted to the national database. Foodborne disease are a global problem and infections in one part of the world may originate on a different continent. Therefore, active surveillance system supports the establishment of similar networks in other regions with the goal to enable real-time comparison of subtyping results form the different networks. The PFGE system will also be used as a primary tool in preparedness and response to acts of foodborne disease outbreaks.