순무싹이 고지방식이를 급여한 흰쥐의 지질대사에 미치는 영향

Abstract

This study was carried out to analyze the major physiochemical components of Brassica rapa (BR) sprouts. In addition, the effect of BR sprouts on serum, liver and adipose tissue lipid metabolism in rats fed high fat diet for 4 weeks for induction hyperlipidemic model rat were also investigated. The proximate compositions of BR sprouts as dry matter basis were that the moisture content 16.35%, 22.51% of crude protein, 21.60% of crude lipid, 4.35% of crude ash, and 35.19% of carbohydrate, respectively. Analysing total amino acids, 18 kinds of components isolated from BR sprouts. The essential amino acid contained in BR sprouts accounted for 47.00% of total amino acid, while the non-essential amino acid accounted for 53.00%. The major unsaturated fatty acids of total lipids were linoleic acid. The ratio of polyunsaturated fatty acids to saturated fatty acids (P/S ratio) was 0.54. The contents of vitamin A and vitamin E were 0.09 mg% and 3.06 mg% respectively. Tartaric acid was the major organic acids. Among the minerals in dried BR sprouts, the content of potassium was the highest (882.50 mg%) and those of magnesium and calcium were comparatively high (342.85 mg%, 274.30 mg%). The main component of free sugars was glucose. The results of effect of BR sprouts on lipid metabolism in rats fed high fat diet were as follows; Weight-matched male Sprague-Dawley rats were assigned to four groups according to dietary fat levels (10% or 20% of diet wt.). Experimental groups were normal diet group (N), high fat diet group (HF), high fat diet with 5% BR sprouts powder group (HF-BRL), and high fat diet with 10% BR sprouts powder group (HF-BRH). The body weight gain was increased in HF group, but gradually decreased to the corresponding level of the N group by fed BR sprouts powder. The concentrations of serum LDL-cholesterol, atherogenic index and cardiac risk factor were tended to be decreased in the BR sprouts powder fed groups compared with the HF group. However, HDL-cholesterol concentration and HDL-C/TC ratio in serum decreased in the HF group and markedly increased in the BR sprouts powder fed groups. Concentrations of triglyceride and total cholesterol in liver were also markedly decreased in the BR sprouts powder fed groups. Triglyceride concentrations of epididymal and mesenteric adipose tissues in the BR sprouts powder fed groups were also decreased compared with the HF group. These results indicate that lipid metabolism in rats fed high fat diet was suppressed by feeding of BR sprouts powder.