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Abstract

ABSTRACT

Preparation and characterization of high tensile strength antimicrobial biodegradable film using water soluble polymer

Kim, Maeng Su Advisor : Prof. In Hwa Lee, Ph.D. Department of Environmental Engineering, Graduate School of Chosun University

Methyl cellulose nanocomposite films plasticized with PEG(polyethylene glycol) were prepared by solvent casting process under addition of 0.025～1 wt% ampicillin and 0.1～1 wt% streptomycin as a antimicrobial agent. Thickness of the prepared films were varied with viscosity of the methyl cellulose (15cp, 400cp, 1500cp). Tensile strength of methyl cellulose films were 15～22 N/mm2. Antimicrobial activities of containing ampicillin and streptomycin methyl cellulose films through the disc diffusion test for the Staphylococcus aureus (KCCM 12214) and Escherichia coli (KCCM 11234), which was known as typical food putrefactive bacteria. The antimicrobial activity of containing ampicillin methyl cellulose films were higher than that of containing streptomycin methyl cellulose films. Antimicrobial PVA(polyvinyl alcohol) films with biodegradability and biocompatibility were prepared with antibiotic additives such as ampicillin and streptomycin. The variations of the disperson, mechanical properties (tensile strength, elongation) of the antimicrobial PVA (degree of polymerization : 1700, saponification : 98～99.5 %) films with antibiotic additives within 1 wt% were prepared and examined. Antimicrobial activities of prepared films containing ampicillin, streptomycin were examined through the disc diffusion test for the Staphylococcus aureus and Escherichia coli. In case of Staphylococcus aureus the clean zone diameters were 23.52～33.52 mm, 13.2～15.52 mm in ampicillin partition layer, streptomycin partition layer respectively. In case of disc diffusion test for Escherichia coli the clean zone diameters were 13.6～15.36 mm in ampicillin partition layer, streptomycin partition layer respectively. Tensile strength of PVA(15 wt%) film were 69～75 N/mm2, and the tensile stregth of the antimicrobial films were decreased linearly with increasing the antibiotic loading amount up to 1 wt%. PVA crosslinking films were prepared by various contents by using glyoxal. Variation of the physicochemical properties of the PVA(degree of polymerization : 1700, saponification : 98～99.5 %) films with glyoxal content in the range 2～10 % were investigated through to FT-IR, DSC, TGA, XRD and UTM. From the result of DSC, it was known that the glyoxal contents increased, the melting point and crystallization temperature of PVA/glyoxal films decreased. TGA analysis indicated that PVA/glyoxal films were thermally more stable than pure PVA film due to some interactions between two component in the PVA and glyoxal. Tensile strength of pure PVA(15 wt%) film were 72 N/mm2, and the tensile strength of the PVA/glyoxal films were 83.5 N/mm2 increased with glyoxal contents. The PVA/glyoxal film prepared with 8% glyoxal shows high tensile stregth and thermally stable characteristics.