비치성 악안면 감염 병소에서 16S rDNA를 이용한 세균의 동정 및 항생제 감수성 검사

Abstract

Purpose: The purpose of this study was to isolate and identify bacteria from the four patients with non-odontogenic infectious lesions ( mucormycosis, chronic inflammation from wound infection and two actinomycosis), and determine their antimicrobial susceptibility against eight antibiotics. Materials and methods: Bacterial culture was performed under three culture conditions (anaerobic, CO2, and aerobic incubator). And the identification of bacteria was performed by 16S rRNA gene (16S rDNA) sequence comparison analysis method. For the sensitivity of the bacteria against eight antibiotics; penicillin G, amoxicillin, tetracycline, cefuroxime, erythromycin, clindamycin, vancomycin and Augmentin？？ (amoxicillin + clavulanic acid), minimum inhibitory concentration (MIC) was evaluated using broth microdilution assay. Result: Nosocomial pathogens such as Enterococcus faecalis, Klebsiella pneumoniae, Bacillus subtilis, and Neisseria flavescens were isolated from mucormycosis. Veillonella parvula, Enterobacter hormaechei, and Acinetobacter calcoaceticus, which are also nosocomial pathogens, were isolated from chronic inflammatory lesion. Actinomyces massiliensis was isolated from actinomycosis in parotid gland. Capnocytophaga ochracea was isolated from actinomycosis in buccal region in anaerobic condition. There was no susceptible antibiotic to all bacteria in mucormycosis. Tetracycline was susceptible to all bacteria in chronic inflammation. C. ochracea was resistant to vancomycin, and penicillin G and other antibiotics showed susceptibility to all bacteria in actinomycosis. Conclusion: The results indicated that the combined treatment of two or more antibiotics is better than single antibiotic treatment in mucormycosis, and penicillin is the first recommended antibiotic to treat actinomycosis.

kew word: mucormycosis, actinomycosis, 16S rDNA