NIH3T3세포 이동과정에서 Dynamin II의 기능

Abstract

Dynamin has been reported the responsibility to formation of nascent vesicle in the endocytic and secretory pathways. Previously study, we shown in the Ras transformed NIH3T3 cells that Dynamin II acts as an intermediate messenger in the Ras signal transduction pathway leading to membrane ruffling and cell migration. Also, the platelet-derived growth factor treatment induced Dynamin II interacted with Myosin II in NIH3T3 cells. However, these results were not enough the evidence for relating to between Dynamin II and Ras signal transduction pathway leading to membrane ruffling and cell migration. Therefore, immunoprecipitation was carried out to identify the potential relationship between Dynamin II interacted with Myosin II and other Ras signaling molecule relating of cell migration such as PI3K and FAK. Our resultㄴ showed that the Dynamin II association with Myosin II as a signaling molecule involved in NIH3T3 cell migration through the Ras/PI3K signaling pathway and this is association with p85 subunit of PI3K. Also, FAK is highly expressed with Myosin II in NIH3T3 (Ras) compared with NIH3T3 cells and lower expressed with Dynamin II. Confocal microscopy also showed the co- localization between Dynamin II and paxillin by PDGF stimulation in NIH3T3 cells. Also, Dynamin II was co-localized with actin filament in immunofluorescence results. After stimulated or not PDGF and treatment of actin inhibitor such as cytochalasin D in NIH3T3 cells have shown that Dynamin II with Myosin II complex inhibited binding to the actin. This result suggest that Dynamin II was localized in focal adhesion when triggered cell migration and bind to actin filament component.

KeyWords:Dynamin II, Myosin II, Ras signal, cell migration, platelet- derived growth factor (PDGF), NIH3T3 cells