MDPC-23 세포분화에서 MTA의 효과

Abstract

Effects of MTA on MDPC-23 cell differentiation Lee, Baek-Hee Advisor: Kim, Heung-Joong, D.D.S., M.S.D., Ph.D. Department of Dental Engineering, Graduate School of Chosun University

Mineral Trioxide Aggregate(MTA) shows strong alkalinity and has similar function with calcium hydroxide. It was developed as root end filling material, and used as pulpotomy and pulp-capping material. However, the effect of MTA on odontoblasts differentiation has not yet been known. The purpose of this study is to determine whether MTA promotes differentiation and nodules formation in MDPC-23 pre-odontoblast cell line. MDPC-23 cells were cultured for up to 15 days in growth media containing differentiation medium with MTA or without MTA. Cultures were stained with Alizarin-S. The expression of OC, ALP, BSP, Type I collagen and SPARC mRNAs were analyzed by RT-PCR. After Alizarin Red-S staining, MDPC-23 cells including MTA at day 5 showed obvious mineralization as compared with control cultures. MTA significantly increased the expression of OC, ALP and BSP mRNAs in MDPC-23 cells as compared with control groups. However, the expression of Type I collagen and SPARC mRNAs in MTA groups decreased slower than control groups. These results indicated that MTA was capable of promoting MDPC-23 cells differentiation and also suggested that MTA might play an important role in dentin mineralization.