사람 치주인대섬유모세포에서 MMP-1, TIMP-1,2, Type 1 collagen, fibronectin 및 UNCL 발현에 미치는 H2O2와 clorhexidine의 효과

Abstract

Inflammatory periodontal diseases are often related to activated phagocytosing leukocytes and free radical oxygen production. Chlorhexidine (CHX) is effective in inhibiting dental plaque accumulation and inflammation. Whether the host antioxidant defenses could benefit from chlorhexidine was not clearly revealed yet. The purpose of this study was to evaluate the effects of chlorhexidine and H2O2 on matrix metalloproteinase-1 (MMP-1), tissue inhibitor of metalloproteinase (TIMP-1, TIMP-2), Type 1 collagen, fibronectin and UNCL expressions in human periodontal ligament fibroblasts (hPDLF). 0.12%, 0.012% and 0.0012% CHX and 0.003%, 0.0003% and 0.00003% H2O2 and mixture of CHX and H2O2 were applied to hPDLF for 1 min and 30 min. The mRNA expressions of MMP-1, TIMP-1 and 2, Type 1 collagen, fibronectin and UNCL in hPDLF were analysed by RT-PCR. The result were as follows: 1. The expression of UNCL mRNA was higher than that of other mRNAs. 2. 0.0012% CHX increased mRNA expressions of hPDLF as application time increased. 3. H2O2 lower than 0.003% increased expression of UNCL mRNA, and did not decrease mRNA expression of hPDLF. 4. hPDLF treatment with 0.12% CHX (with or without H2O2) resulted in no gene expression. 5. hPDLF treatment with 0.012% CHX (with or without H2O2) for 30 minutes resulted in no gene expression In conclusion, because low concentration of CHX and H2O2 increased UNCL mRNA expression of hPDLF, low concentraction of CHX and H2O2 may have an antioxidative effect.