배양망막색소상피세포에서 산화성스트레스에 의한 IL-8의 발현변화
- Author(s)
- 허준
- Issued Date
- 2008
- Keyword
- Interleukin-8|Monocyte chemoattractant protein-1|N-acetylcys teine|Oxidativestress|Paraquat
- Abstract
- Purpose: To evaluate the expression of Interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) in the cultured ARPE-19 cells.
Methods: ARPE-19 cells were exposed to paraquat for 72 hours and then assayed for mRNA expression of IL-8 and MCP-1 by reverse transcription polymerase chain reaction method. And the effect of antioxidant, N-acetyl cysteine (NAC) on the oxidative stressed - ARPE-19 cells was measured.
Results: IL-8 expression was increased in the oxidative stress group compared with the control, but the increase of expression was blocked by mixing NAC with paraquat.
Conclusion: Oxidative stress stimulates IL-8 expression in the cultured human retinal pigment epithelial cells, which could be helpful to understand the mechanism of neovascularization in age-related macular degeneration.
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- Embargo2008-07-18
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