배양망막색소상피세포에서 산화성스트레스에 의한 IL-8의 발현변화

Abstract

Purpose: To evaluate the expression of Interleukin-8 (IL-8) and monocyte chemoattractant protein-1 (MCP-1) in the cultured ARPE-19 cells. Methods: ARPE-19 cells were exposed to paraquat for 72 hours and then assayed for mRNA expression of IL-8 and MCP-1 by reverse transcription polymerase chain reaction method. And the effect of antioxidant, N-acetyl cysteine (NAC) on the oxidative stressed - ARPE-19 cells was measured. Results: IL-8 expression was increased in the oxidative stress group compared with the control, but the increase of expression was blocked by mixing NAC with paraquat. Conclusion: Oxidative stress stimulates IL-8 expression in the cultured human retinal pigment epithelial cells, which could be helpful to understand the mechanism of neovascularization in age-related macular degeneration.