두경부 편평세포암종에서 PLK1과 HSF1의 DOUBLE KNOCKDOWN에 의한 항암효과

Abstract

Elevated level of PLK1 was reported in many different types of cancer cells and PLK1 has been considered as a novel prognostic marker for several tumor types. Recent studies have also reported the aberrant expression of HSF1 in human cancer of various origins correlates with the development of cancer, invasiveness, and metastasis. In this study, we investigated the effect of PLK1 and HSF1 double knockdown in oral squamous cell carcinoma using the small interfering RNA technique. The high level of PLK1 and HSF1 was observed in human oral squamous cell carcinoma compared with normal tissue. Silencing of both PLK1 and HSF1 showed a synergic reduction of cell viability, induction of cell death, and loss of adhesion function compared with PLK1 single depletion. Silencing of PLK1 reduced the activity of the G2/M phase regulator Cdc2/CyclinB complex. Interestingly, decreased level of PLK1 led to the inhibition of Hsps expression via the down-regulation of HSF1. These findings provide the potential value of PLK1 and HSF1 as a target for oral cancer therapy.