Chlorine e6 유도 광역학 치료에 의한 RK3E-ras 세포의 Apoptosis 유도

Abstract

Photodynamic therapy (PDT), a promising cancer treatment that employs a combination of a photosensitizing chemical, oxygen, and visible light, induces apoptosis in cancer cells. The mechanisms of its anticancer activity include generation of singlet oxygen and reactive oxygen species that directly damage cancer cells. Chlorine e6 is a newly introduced photosensitizer which is reduced forms of porphyrins for use in PDT. In the current study, the author evaluated the generation of reactive oxygen species and the mode of cell death of RK3E-ras cells induced by photosensitization with chlorine e6. RK3E-ras cells were incubated with chlorin e6 for twenty four hour. Chlorine e6-induced PDT was generated reactive oxygen species and led to significant RK3E-ras cell growth, as assessed by MTT assay. Apoptotic pattern was clearly demonstrated by TUNEL assay and flow cytometry. Subsequently, PDT with chlorine e6 induced activation of apoptosis related proteins such as caspase-3/-7, cytochrome c, and PARP. In contrast, cell survival factor Bcl-2 was reduced following PDT. These results demonstrate that chlorine e6-mediated PDT induces apoptosis through the generation of reactive oxygen species upon light activation. Chlorine e6 is a potent photosensitizer with the capacity to directly initiate apoptosis by acting upon mitochondria.