태반조직으로부터 유래한 다분화능 줄기세포의 분리 및 특성화

Abstract

It is becoming increasingly evident that human adult somatic tissues can serve as a reliable source of multipotent stem cells. In this study, we isolated the fibroblast-like cells from human placenta tissues and chracterized it using the FACS analysis. We confirmed the multipotentiality of cellular differentiation from placenta fibroblast-like cell into bone, adipose tissue and neuron. Placenta-derived clones were highly proliferative and immunophenotypically positive for CD13, CD29, CD44, CD54, CD73(SH3), CD90, CD105(SH2) and HLA class I, but negative for CD14, CD34, CD45 and HLA-DR. Incubation of these MSC with osteogenic agents resulted in development of osteocytes. Under proadipogenic conditions, several oil vacuoles appeared in the cells. Exposure of cells to basic fibroblast growth factor resulted in neural differentiation. Adipogenic, osteogenic and neurogenic differentiation demonstrated a multilineage capacity of the cells comparable with bone marrow derived MSC. By considering ease in collection, banking and availability, placenta-derived multipotent stem cells might offer an added therapeutic advantage compared to marrow. In addition, these multiliniage cells unlike bone marrow cells, have a lower precursor frequency and a lower level of bone marrow antigen expression, and lack neural antigens. Altogether, the placental derived multipotent cells are intriguing as a potential source of cells for cellular therapeutics for stromal, bone and neural repair.