전기방사을 이용한 항균 나노 섬유제조에 관한 연구

Abstract

The electrospinning technique has been actively explored recently for potential applications because that offers ultrafine fiber, high specific surface area and possibility of various modification in spite of simple process. We made fiber out of the polymer solution which added Ginkgo biloba extraction, propolis, silver, streptomycin to cellulose acetate/acetone solution through the electrospinning. Electrospinning conditions was as follows; 100㎕/min of flow rate, and 10cm of tip-to-collector distance(TCD), applied voltage was 12kV. After electrospinning process, fiber morphology was observed from Scanning Electron Microscope (SEM). SEM photographs showed that average diameter of fibers by prepared via electrospun was approximately 800nm, 600nm, 600nm, 200nm in cellulose acetate fibers, Ginkgo-cellulose fibers, streptomycin-cellulose fibers, silver-cellulose fibers. Antimicrobial activities of cellulose acetate fiber from added Ginkgo biloba extraction, propolis, silver, streptomycin was investigated by disc diffusion test and optical density test against S. aureus and E. coli. In case of disc diffusion test of S. aureus, the clean zone diameter was increase according to content of antimicrobial additive. Also, optical density test showed that it appeared to same results. Bacteria reduction of ginkgo-cellulose fibers, streptomycin-cellulose fibers, silver-cellulose fibers, propolis-cellulose fiber was 0.5wt% silver-cellulose fibers > 0.5wt% streptomycin-cellulose fibers > 20% propolis-cellulose fiber > 30wt% ginkgo-cellulose fibers for S. aureus by optical density test during 24 hours. However, bacteria reduction was 20% propolis-cellulose fiber > 0.5wt% silver-cellulose fibers > 30wt% ginkgo-cellulose fibers > 0.5wt% streptomycin -cellulose fibers after 24 hours. Streptomycin-cellulose fiber was 98% of bacteria reduction during 24 hours. But, antimicrobial effect of streptomycin rapidly decrease after 24 hours.