아위버섯 균사체 심부배양 특성 및 기능성 연구

Abstract

This study examines features and functions of mycelial submerged culture of Pleurotus ferulae. Its four strains (F-1, F-2, F-3 and F-4 ) collected domestically are publicly announced to select good strains and the best strain, F-2, is selected as its mycelial grows fast and density is good. The strain of P. ferulae selected shows the best growth in MYPA medium and as the growth is the best at 25℃, it is demonstrated that the optimal temperature us 25 ℃ and optimal acid degree is pH 6.5. The optimal culture volume at the flask culture is 50mL at the 300ml flask , and shaking speed is 150 rpm. This study examines optimal cultural conditions for mycelial growth and production of exo-polysaccharide, and finds that the effective culture days and innoculation amount of the optimal innoculation source are seven days and 5% innoculation(w/v). The factor of nutritial need is one of prerequisites for production of matter containing microbes and effective components. However, as there are few studies on the factor of nutritial need for mycelial growth of P. ferulae and exo-polysaccharide, this study begins batch culture to examine the factor. As a result, the optimal growth medium should be made of 5% of glucose (w/v) for carbon source, 1.0% of polypeptone (w/v) and 0.8% of yeast extract(w/v). The optimal agitation speed and ventilation of 5 L jar fermentation are 200rpm and 1.5vvm. When this study conducts a batch culture under the optimal culture condition designed from the flask with 25℃ of temperature, pH 6.5, 200rpm of agitation speed, and 1.5 vvm of ventilation amount for 12 days, at the tenth days of the culture, it obtains the largest hypha of 10.01 g/L and 3.94 g/L of exo-polysaccharide, and the production of the exo-polysaccharide is closely related to hypha growth, which indicates a multiplicative interlocking. As fruit body and complex of the mycelium has more toxicity to the cell line of lung cancer rather than that of uterine cancer, the complex abstracted contains ani-carcinogenic materials and MTT can be effectively used for search of its functionality. Water extracts from the fruit body of P. ferulae controls muliplication of the spleen cells even at the 200 times-diluted concentration and the complex abstracted from the mycelium controls multiplication of the spleen cells even at the 300 times-diluted concentration. Water extracts from the fruit body of P. ferulae controls production of TBA responsive matter by hydrogen peroxide at the 200 times-diluted concentration, and the complex abstracted from the mycelium controls it even at the 400 times-diluted concentration. However, the result obtained through this study is not sufficient to develop effective processing for production of mycelial growth and exo-polysaccharide. Therefore, for mass production of mycelium and exo-polysaccharide, we need further studies on features of exo-polysaccharide produced from the mycelium, optimization of culture conditions, preparation and design of proper effector. And it is demonstrated that the complex abstracted from the P. ferulae has greater toxicity to the lung cancer cells and remarkably increases toxicity to lung cancer and uterine cancer cells. As the P. ferulae is edible and its complex abstracted is thought to have low toxicity, the complex can be used as a material to enhance anti-cancer action and for more scientific and organized studies, the effective matter from the P. ferulae should be separated and refined to identify its active mechanism and secure stability through research on toxicity.