마타리(Patrinia scabiosaefolia F.)로부터 새로운 Iridoid계 화합물 분리 및 규조규명

Abstract

Patrinia scabiosaefolia (Valerianaceae) is a perennial herb and an endemic species in Korea. The genus Patrinia is taxonomically classified into four species, P. saniculaefolia,P. scabiosaefolia, P. villosa, and P. rupestris.3) This genus is one of the valuable crude drugs which has been used in Korea and China as a traditional folk medicine for the treatment of initial stages of edema, appendicitis, endometriosis and inflammation.4) Saponins, coumarins, iridoids,and flavonoids isolated from this genus have demonstrated sedative, antibacterial, and cytotoxic effects, In an ongoing investigation into anti-proinflammatory compounds from natural products, the methylene chloride soluble fraction of P. scabiosaefolia F was found to inhibit hIL-6 production in TNF-α stimulated human osteoblast-like MG-63cell line. Bioassay-guided isolation of this fraction led to the purification of three-new iridoid compounds, and two-lignan compounds. Three new iridoid gave a name to PatiridoidⅢ(1), PatridoidⅣ(2), PatridoidⅤ(3). compound1 has two-isovaleryoxy group and C-8 has methoxyl group and C-10 has oxygen-bearing methylene also compound2 has two-isovaleryoxy group and C-10 has oxygen-bearing methyene group. compound3 have three methoxyl group that located C-1, C-3, C-11. For that this reason they are considered as new compounds. Compound4, Sesquilignan of Lappaol E, already isolated from Arctium, this compound have three aromatic rings, carbonyl carbone, and oxymethin-carbone. Compound5, Dibenzylbutyrolactone lignan - Nortrachelogenin. This compoumd has been isolated another plant. their structure were determined by 1H-NMR, 13C-NMR, HMBC, HSQC and NOESY. all of there compounds pure form. In vitro anti-inflammatory activity was evaluated by inhibition(%) of hIL-6 production in TNF-α stimulated MG-63 cell line. Compound 5, isolated from the CH2Cl2 fraction decreased to 66.1± 2.5%.