노화에 따른 신경계의 유전자 손상복구 인자의 변화에 대한 연구

Abstract

Changes of DNA repair factors with age in nervous system There is a rapid loss of weight with age in human brain. That is related to the reduction of neuronal bodies, the loss of dendritie, the accumulation of mitochondrial damage, and the reduction in DNA repair activity. Once DNA sequence is lost, perfect recovery is impossible. This character of the DNA makes genetic fragments a major target for age-related decline. Cells comprise intricate DNA repair system to avert DNA damage. DNA repair system may itself be injured by age-related celluar process including reactive oxygen species. In this study we will survey the changes in DNA repair factors during aging in rat, including mismatch repair (MMR), base excision repair (BER), and double-strand break (DSB) repair. This paper shows that the level of Msh2 protein declines after 1 year of age significantly, which is one of the key enzyme involved in MMR. BER factor, MTH1 and OGG1 also are slightly reduced with age. In contrast to increased DSB damage(γ-H2AX), the repair factors for DSB, Ku80 and DNA-PKCs, are reduced in aging rat brain. These results suggest that the increased DNA damage and the reduction of several DNA repair factors with age may play significant role in deterioration of neural system.

Key-wards : DNA repair facors, DNA damage, Brain, aging