UVB에 손상된 생쥐의 피부에 미치는 자초 (Lithospermum erythrorhizon) 추출물의 효과

Abstract

This study was intended to identify the effectiveness of Lithospermum erythrorhizon in the UVB-damaged mouse skin. The C57BL mice that weighted about 18 g were divided into three groups; the control group (A group), the UVB irradiated group (B group), and the group treated with 0.5% Lithospermum erythrorhizon emulsion after UVB irradiation (C group). Lithospermum erythrorhizon was massaged on finger the shaved skin for 3～5 second. 10 mouses were collected and sacrificed at 24 hrs, 48 hrs, 72 hrs, 120 hrs, and 168 hrs, respectively. The values of the transepidermal water loss (TEWL) were observed by Tewameter TM 300 (Courage + Khazaka electonic GmbH, Germany), melanin values and erythema index by Mexameter MX 18 (Courage + Khazaka electonic GmbH, Germany). The morphological changes of the skin tissues were observed by the light micrograph (ZEISS, Germany), the scanning electron micrograph (Hitachi S-4800, Japan), the transmission electron micrograph (JEOL, 2000 FX-Ⅱ형, Japan). The superoxidase dismutase (SOD) activity were observed by the Beauchamp and Fridovin's method and the catalase (CAT) activity by the Aebi's method. In the result of transepidermal water loss (TEWL), the C group was decreased than B groups by time. At the 168 hrs group was significantly lower (p < 0.05). In the result of melanin value, the C group was decreased than B group, but meaningless (p > 0.05). In the result of erythema index, the C group was meaningfully lower at 24 hrs, 48 hrs, and 72 hrs group than B group (p < 0.05). In the results of SOD activities, the C group was meaningfully lower at the 48 hrs, 120 hrs, and 168 hrs group than B group (p < 0.05). In the result of CAT activities, the C group was decreased at the 120 hrs and 168 hrs group, but meaningless (p > 0.05). In the result of light micrograph observation, the B group observed sunburn cells in the epidermis and acute inflammation in the dermis at the 24 hrs and 48 hrs. And proliferation of the epidermis and the stratum granulosum, and found the hyperkertosis at the 72 hrs, 120 hrs, and 168 hrs group than C group. The C group alleviated inflammation in the dermis than B group at the 24 hrs and 48 hrs. And inhibited the proliferation of the epidermis at the 72 hrs, 120 hrs, and 168 hrs groups than B group. In the result of scanning electron micrograph observation, the B group was swelling in epidermis surface at the 24 hrs, seemingly dry skin surface at the 48 hours, irregular plate shape and fine wrinkles at the 72 hrs, founded scab at the 120 hours, and the overlapped surface with the irregular plate shape without any fine wrinkle at the 168 hrs. The C group was allevited swelling than B group at the 24 hrs, formation of the scab at the 48 hrs, regular plate shap at the 72 hrs, new keratin observated at the 120 hrs partially, and fine fiber covered epidermis surface at the 168 hrs. In the result of transmission electron micrograph observation, the B group was disruption of lipid layer and increased lamellar bodies at the 24 hrs, exocytisis of lamellar bodies at the 48 hrs, increased lamellar bodies at the 72 hrs, formation new lamellar bodies at the 120 hrs and 168 hrs. The C group was facilitation of increased lamellar bodies and reformation lamellar bodies than B group at the all groups. Almost all the structures were recovered at the 168 hrs group. The results of all the tested after UVB irradiation were better the C group than B group. As shown in this study, therefore, the application of the Lithospermum erythrorhizon treatment to the UVB-damaged skin can be effective in treatment for the damaged skin from the viewpoint of national health care or alternative medicine.