Neuroprotective effects of Tripterygium regelii extract in human neuroblastoma SH-SY5Y cells
- Issued Date
- 2007
- Abstract
- ABSTRACT
Neuroprotective effects of Tripterygium regelii extract in human neuroblastoma SH-SY5Y cells
Bong-suk,Choi
Advisor: Prof. Sung-Jun Kim Ph. D.
Department of Biotechnology,
Graduate School of Chosun University
The reality is facing a rapidly aging society in Korea. It means that the aging population will increase continuously. Consequently, geriatric nervous disease Patients also will increase continuously. The nervous diseases are Alzheimer's disease (AD), The Parkinson's disease (PD) and schizophrenia.
PD is characterized pathologically by loss of dopaminergic neurons and low response of dopaminergic neurons in the substantia nigra (SN), but still not entirely clear. The reason of disease is rising on oxidative injure, environmental factors and genetic predisposition as co-factors of the neurodegenerative process.
Tripteryguim regelii Sprague et Takeda is a very general plant at nature. The T. regelii has long been used as an adaptogen in traditional Korean medicine. In this study, the extracted from ethanol (TRE) and methanol (TRM) of T. regelii were evaluated using various anti-oxidants assays and neuroprotective effects.
- Alternative Author(s)
- Bong-suk,Choi
- Affiliation
- 조선대학교 자연과학대학
- Department
- 일반대학원 환경생명공학과
- Awarded Date
- 2008-02
- Table Of Contents
- CONTENTS
LIST OF TABLE ⅴ
LIST OF FIGURES ⅵ
ABBREVIATION ⅷ
ABSTRACT ⅹ
I. INTRODUCTION 1
II. MATERIALS AND METHODS
A. Materials 7
B. Methods
1. Sample extraction
a. Preparation of TRE extract 9
b. Analysis of total flavonoids compounds 9
c. Analysis of total phenolic compounds 10
d. Determination of DPPH radical scavenging activity 10
e. H2O2 scavenging activity assay 11
f. Determination of reducing power 11
2. Animals and treatments 12
a. Total RNA extraction 13
b. Reverse transcription (RT)-Complement DNA (cDNA) preparation 14
c. Polymerase chain reaction (PCR) and the analysis of PCR products 14
d. Total protein isolation 16
e. Western blot analysis 16
3. Cell culture and treatment 17
a. Total RNA extraction 18
b. RT-cDNA preparation 18
c. PCR and the analysis of PCR products 19
d. Total protein isolation 19
e. Western blot analysis 20
f. Determination of cell viability 20
g. Determination nuclear staining for morphological changes 21
4. Statistical evaluation 22
III. Result
A. Antioxidant activities in two extracts of TRE
1. Analysis of total flavonoids and phenolic compounds 23
2. Determination of DPPH radical scavenging activity 25
3. H2O2 scavenging activity 25
4. Determination of reducing power 27
B. TH induction of TRE treated rat brain and SH-SY5Y
1. TH and brain-derived neurotrophic factor (BDNF) induction of Rat 29
2. TH and BDNF induction of SH-SY5Y 29
C. Neuroprotective effects of TRE in SH-SY5Y
1. 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction assay 32
2. 4,6-diamidino-2-phenylindole (DAPI) 34
3. Western blot 34
IV. DISCUSSION 37
V. REFERENCE 40
VI. 감사의글 46
- Degree
- Master
- Publisher
- 조선대학교
- Citation
- 최봉석. (2007). Neuroprotective effects of Tripterygium regelii extract in human neuroblastoma SH-SY5Y cells.
- Type
- Dissertation
- URI
- https://oak.chosun.ac.kr/handle/2020.oak/7000
http://chosun.dcollection.net/common/orgView/200000235894
- Appears in Collections:
- General Graduate School > 3. Theses(Master)
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