호주산 propolis의 항균활성과 치주조직에 끼치는 영향

Abstract

Proplolis is a naturally-occurring bee products and has been used in folk medicine for several purposes such as an antiseptic, anti-inflammatory, antimycotic and bacteriostatic agent. The aim of the present work was to evaluate the antibacterial activity and periodontal effects of Australian propolis (Green-One natural health, Sydney, Australia). Antibacterial effect of diluted propolis on seven oral pathogens, Prevotella intermedia (Pi), Prevotella nigrescens (Pn), Porphyromonans endodontalis (Pe), Porphyromonas gingivalis (Pg), Tannerella forsythensis (Tf) Actinobillus actinomycetemcomitans (Aa), and Fusobacterium nucleatum (Fn), were tested and their cytotoxic effect on human gingival fibroblasts (hGF) and periodontal ligament fibroblasts (hPDLF) were evaluated by MTT. Cell viability assays were performed using third to fourth-passage of hGF and hPDGF. And, for investigating the periodontal effect of propolis, gingival creviculr fluid (GCF) and sulcular bleeding index (SBI) were measured 10-day after applying diluted propolis with periodontal pack. MTT assay had demonstrated that 1/2^(7) dilution of propolis had antimicrobial effect and was safe for hGF and hPDLF. After applying diluted propolis with periodontal pack, GCF was significantly reduced but SBI showed decreasing tendency. Author confirms that propolis has antiseptic, antibacterial and antiinflammatory properties if appropriate dilution can be prepared. And, more researches are needed to find out the appropriate diluted propolis solutions which are less cytotoxic for tissue cells but present strong antibacterial properties, and promote the wound healing.