치수와 치은 섬유모세포에서 Mineral Trioxide Aggregate (MTA)가 세포증식, Alkaline phosphatase, Osteonectin 발현에 미치는 영향

Abstract

Mineral trioxide aggregate (MTA) is used clinically for pulp capping, retrograde filling, repair of root perforation, where it stimulates dentin, cementum formation and promotes gingival healing. In this study, the author investigated the in vitro effects of MTA on various gene expression regarding dentinogenesis and cell viability assay in cultured primary human dental pulp and gingival fibroblast cells. The author also examined the effects of this material on cellular alkaline phosphatase activity as a potential indicator of mineralization. For gene expression on MTA, reverse transcriptase polymerase chain reaction was performed using primer sets for glyceral- dehyde-3-phosphate dehydrogenase, alkaline phosphatase (ALP), osteonectin (SPARC), and dentin sialophosphoprotein (DSPP) after 2 and 4 days. Cell viability assay showed that the proportion of MTA treated pulp cells exposed for 5 days to MTA was higher than the control cells. Of the genes investigated in this study, ALP and osteonectin (SPARC) were increased than control. In contrast, MTA did not induce dentinogenic or osteogenic activity. These findings suggest that this culture system may be useful in the future as a model for studying the mechanisms underlying dentin or bone regeneration after the treatment of MTA. Exposure to MTA material did not induce cytotoxic response in the dental pulp and gingival fibroblast cells. In addition, MTA could influence the behaviour of human pulp cells by increasing the ALP activity and SPARC synthesis.