생장조절제 및 배양조건이 은행나무(Ginkgo biloba L.) 잎의 세포배양과 습관성 유도에 미치는 영향
- Issued Date
- 2007
- Keyword
- 생장조절제|배양조건|은행나무|세포배양|습관성 유도
- Abstract
- The influence of various culture conditions on callus growth and maintenance was studied the callus derived from Ginkgo biloba L. leaves, which were grown on Murashige and Skoog (MS) medium supplemented with growth regulators; α-naphthaleneacetic acid (NAA), benzylaminopurine (BA) and kinetin. The optimal concentrations of growth regulator for callus induction were 10μM NAA with 5μM BA or 0.5μM kinetin in the light. In the dark condition, the optimal concentration of growth regulator was 10μM NAA with 0.5μM BA. In the light, MS medium supplemented with 100μM NAA and 5μM BA combination was the optimal for growth and maintenance of callus cultures. The suspension cultures were initiated with 1.5g of the callus derived from Ginkgo leaf disks on MS medium. The optimal concentration of growth regulator for maintenance of callus culture was 10μM NAA with 0.5μM BA or kinetin. To determine the production of the quercetin dehydrate and keamperol profiles from suspension callus was compared by HPLC analysis. In the leaf extract, the contents of quercetin dehydrate and keamperol were 0.072 and 2.24μg/20μl, respectively. The extract of callus contained 0.556 and 0.157μg/20μl, respectively. The free radical scavenging activity of methanolic extracts from induced callus was more effective than leaf extract. The callus extracts concentration of 0.2mg/ml was enough for 50% inhibition. The habituation was more effective in the dark culture condition than in the light one.
- Alternative Title
- Effects of growth regulators and culture conditions during the induction of cell culture and habituation in the leaves of Ginkgo biloba L.
- Alternative Author(s)
- Kim, Jung-Suk
- Affiliation
- 조선대학교 대학원
- Department
- 일반대학원 생물학과
- Advisor
- 박현용
- Awarded Date
- 2007-08
- Table Of Contents
- 1. 서론 = 1
2. 재료 및 방법 = 4
2.1. 식물재료 = 4
2.2. 방법 = 4
2.2.1. Callus의 유도 = 4
2.2.2. 생장조절제의 효과 = 4
2.2.3. 현탁배양 = 5
2.2.4. 생장곡선 = 5
2.2.5. 습관성 callus의 유도 = 6
2.2.5.1. 고체배양에서 습관성 callus의 유도 = 6
2.2.5.2. 현탁배양에 의한 습관성 callus의 유도 = 6
2.2.6. Flavonol glycosides 확인 = 7
2.2.6.1. 시약 및 표준물질 = 7
2.2.6.2. 시료 제조 = 7
2.2.7. DPPH assay = 8
2.2.7.1. 시료제조 = 8
2.2.7.2. DPPH 측정 = 9
3. 결과 및 고찰 = 10
3.1. Callus 배양 = 10
3.1.1. Callus의 유도 = 10
3.1.2. Callus의 형태 = 17
3.1.3. Callus의 유지 = 23
3.2. 현탁배양 = 24
3.2.1. 광 조건 = 24
3.2.2. 암 조건 = 28
3.3. 생장곡선 = 30
3.3.1. 광 조건 = 30
3.3.2. 암 조건 = 34
3.4. 습관성 callus 유도 = 36
3.4.1. 고체배양에서 습관성 callus의 유도 = 36
3.4.2. 현탁배양에 의한 습관성 callus의 유도 = 40
3.4.2.1. 광 조건 = 40
3.4.2.2. 암 조건 = 42
3.5. Flavonol glycosides 확인 = 44
3.6. DPPH assay = 48
4. 결론 = 53
5. 참고문헌 = 55
- Degree
- Master
- Publisher
- 조선대학교 대학원
- Citation
- 김정숙. (2007). 생장조절제 및 배양조건이 은행나무(Ginkgo biloba L.) 잎의 세포배양과 습관성 유도에 미치는 영향.
- Type
- Dissertation
- URI
- https://oak.chosun.ac.kr/handle/2020.oak/6886
http://chosun.dcollection.net/common/orgView/200000234399
- Appears in Collections:
- General Graduate School > 3. Theses(Master)
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생장조절제 및 배양조건이 은행나무(Ginkgo biloba L.) 잎의 세포배양과 습관성 유도에 미치는 영향.pdf
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