대식세포주에서 박테리아성지질다당류 자극에 의한 분비백혈구단백분해효소억제제와 성장인자들의 발현

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Secretory leukocyte protease inhibitor (SLPI) was known that bacterial lipopolysaccharide (LPS)-induced product of macrophage. Macrophages play an important role in the development of inflammatory responses by secreting an array of cytokines and chemokines in a tissue microenvironment. To identify the function and relationship between potent growth factors and SLPI after LPS stimulation, The reverse transcriptase polymerase chain reaction (RT-PCR) and Western blots were performed for the detection of mRNA and protein expression of SLPI and growth factors such as VEGF (vascular endotherial growth factor), PDGF (platelet-derived growth factor), bFGF (basic fibroblast growth factor) after 100ng LPS stimulation on the RAW264.7 cells. The result of RT-PCR was showed SLPI mRNA expression was increased from 60 min to 48 h after incubation with LPS in RAW 264.7 cells. VEGF and PDGF mRNA was expressed highly at initial stage by LPS stimulation. The expressions of bFGF and collagen type I mRNAs showed very weakly after LPS treatment. SLPI protein level was increased likely the mRNA levels in lysates after LPS stimulation. Additionally, the phase contrast and scanning electron microscopic observation showed that the LPS induce the change of morphology of the RAW264.7 cells. From these results, it suggests that the secreted SLPI by LPS treatment may associate with VEGF and PDGF expression in RAW264.7 cells.
Alternative Title
Expression of secretory leukocyte protease inhibitor and growth factors induced by lipopolysaccharide stimulation in RAW264.7 macrophages
Alternative Author(s)
Son, Wook-Hee
조선대학교 대학원
일반대학원 치의학과
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Table Of Contents
Ⅰ. 서론 = 1
Ⅱ. 실험재료 및 방법 = 3
1. 세포주와 배양 = 3
2. 역전사중합효소반응(reverse transcriptase polymerase chain reaction, RT-PCR)과 실시간 역전사중합효소반응(Real-Time PCR) = 3
3. 항체제조 = 5
4. 면역학적 단백질 검출법(Westernblotting) = 5
5. 면역침강법(Immunoprecipitation) = 6
6. 광학현미경 및 주사전자현미경적 관찰 = 6
Ⅲ. 실험 결과 = 8
1. LPS 자극을 준 RAW264.7 세포에서 SLPI와 성장인자들의 mRNA 발현 = 8
2. LPS 자극을 준 RAW264.7 세포의 세포질과 배양액에서 SLPI 단백질발현 = 8
3. LPS 자극에 따른 RAW264.7 세포의 형태학적 변화 = 9
Ⅳ. 총괄 및 = 고안11
Ⅴ. = 결론16
참고문헌 = 17
사진부도 설명 = 23
조선대학교 대학원
손욱희. (2007). 대식세포주에서 박테리아성지질다당류 자극에 의한 분비백혈구단백분해효소억제제와 성장인자들의 발현.
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General Graduate School > 4. Theses(Ph.D)
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