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Pycnogenol(PYC)에 의한 Human Fibrosarcoma Cells의 DNA 손상에 따른 항산화제의 prooxidant 역할

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Author(s)
박연선
Issued Date
2007
Abstract
Park Yeon-sun
Advisor : Prof. Young-Gon Kim, Ph.D
Department of Biology,
Graduate School of Chosun University

Flavonoids are a group of naturally occurring phenolic compounds which are widely distributed in nature. Although, many dietary flavonoids have been suggested to be beneficial for health as an antioxidants against oxidative stress, however, a number of studies show that these can also exhibit prooxidant role under certain condition. Pycnogenol (PYC), polyphenolyic compounds with antioxidative activity, acted as a prooxidant causing a copper and iron dependent DNA damage in human fibrosarcoma cells (HT1080). Treatment of DNA from plasmid pBR322 and human fibrosarcoma cells with PYC plus copper and iron caused strand scission and the formation of 8-hydroxy-2’-deoxyguanosine (8-OHdG) in DNA. DNA strand breaks and formation of 8-OHdG were closely related to the metal ions and PYC concentrations. On the contrary, PYC further did not induce apoptosis of HT 1080 cells at higher concentrations of PYC in the presence of transition metals in cells, but rather protected cells from prooxidant-induced apotopsis. These results imply that intracellular metal ions induced reactive oxygen species and may have dependent upon the concentrations of antioxidants for protection in the oxidative cleavage of DNA and apoptotic cell death.
Alternative Title
Role of Prooxidant on DNA Damage of Human Fibrosarcoma Cells by Pycnogenol(PYC)
Alternative Author(s)
Park Yeon-sun
Affiliation
일반대학원 생물학과
Department
일반대학원 생물학과
Advisor
김 영 곤
Awarded Date
2007-08
Table Of Contents
Ⅰ. 서 론‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ 1Ⅱ. 재료 및 방법‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ 131. 시 약 ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ 131) 항산화제 및 금속이온‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ 132) 세포 배양 및 추출‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ 133) HPLC와 ELISA‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ 142. 금속이온과 항산화제에 의한 DNA 손상‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐153. Pycnogenol의 Fibrosarcoma에서의 세포생존률 측정161. Animal cell culture‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ 161) 배양액 제조‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ 162) Serum inactivation‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ 163) cell plating‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ 174) Cell Maintaining‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ 172. MTT와 XTT‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ 184. 항산화제의 HPLC와 ELISA를 통한 8-OHdG 생성확인‐ 191. HPLC‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ 192. ELISA‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ 20Ⅲ. 결 과‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ 221. 금속이온과 항산화제에 의한 DNA손상정도‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ 22
2. Pycnogenol의 Fibrosarcoma에서의 세포생존률 측정‐ ‐ ‐263. HPLC와 ELISA를 통한 8-OHdG 생성 확인‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐28Ⅳ. 고 찰‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐33Ⅳ. 참 고 문 헌‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐ ‐37
Degree
Master
Publisher
조선대학교
Citation
박연선. (2007). Pycnogenol(PYC)에 의한 Human Fibrosarcoma Cells의 DNA 손상에 따른 항산화제의 prooxidant 역할.
Type
Dissertation
URI
https://oak.chosun.ac.kr/handle/2020.oak/6830
http://chosun.dcollection.net/common/orgView/200000234317
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General Graduate School > 3. Theses(Master)
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