한국산 솜대의 항암효과 및 면역조절 기능 연구

Abstract

The effect of acetone fraction prepared from bamboo leaf on cell differentiation was investigated in a HL-60 cell culture system. Treatment of HL-60 cells with 50-400㎍/㎖ actone fraction of bamboo leaf for 72h inhibited cell proliferation and induced a small increase in cell differentiation. Interestingly, synergistic induction of HL-60 cell differentiation was observed when the acetone fraction of bamboo leaf was combined with either 5nM 1,25-(OH)2D3 or 50nM all-trans retinoic acid. Flow cytometric analysis indicated that combinations of 1,25-(OH)2D3 and acetone fraction of bamboo leaf stimulated differentiation predominanatly to monocytes whereas combinations all-trans retinoic acid and the acetone fraction of bamboo leaf stimulated differentiation predominantly to granulocytes. These results suggest that the acetone fraction of bamboo leaf enhanced leukemia cell differentiation and suggest a possibility of bamboo in the treatment of leukemia. Also, We investigated the effects of acetone fraction prepared from bamboo leaf on the production of IL-12 from mouse macrophages stimulated with lipopolysaccharide(LPS). The acetone fraction of bamboo leaf potently inhibited the LPS-induced IL-12 production from RAW 264.7 monocytic cell-line in a dose-dependent manner. The effect of the acetone fraction of bamboo leaf on IL-12 gene promoter activation was analyzed by transfecting RAW 264.7 cells with IL-12 gene promoter/luciferase constructs. The repressive effect mapped to a region in the IL-12 gene promoter containing a binding site for NF-κB. Futhermore, activation of macrophage by LPS resulted in markedly enhanced binding activity to the NF-κB site, which significantly decreased upon addition of the acetone fraction of bamboo leaf, indicating that the acetone fraction of bamboo leaf inhibited IL-12 production in LPS-activated macrophages via inhibition of NF-κB binding activity.