자외선에 의해 유도되는 Matrix Metalloproteinase 활성 및 발현에 대한 apigenin과 luteolin의 저해 효과

Abstract

Exposure of ultraviolet (UV) light for long term and repeated on the skin often induces chronic skin diseases such as skin cancer as well as photoaging, and the mechanisms of these skin damages are closely associated with up-regulation of collagenases activities. Moreover, UV-induced collagenases cause connective tissue damage and the skin to become wrinkled and aged. This study investigated the effect of apigenin and luteolin on the ultraviolet A (UVA)-induced matrix metalloproteinases (MMP-1 (collagenase-1) and MMP-13 (collagenase-3)) expression in HaCaT human keratinocytes. Treatment with apigenin and luteolin decreased the UVA-induced generation of reactive oxygen species (ROS). In addition, apigenin and luteolin significantly suppressed the UVA-induced expression of MMP-1 and -13 mRNA. Moreover, transient transfection assay using activator protein-1 (AP-1)-linked luciferase reporter gene assay revealed that apigenin and luteolin reduced UVA-induced AP-1 transcriptional activity. In this study investigated the effects of apigenin and luteolin on UVA-induced c-Jun and c-Fos expression in HaCaT cells. Apigenin and luteolin inhibited UVA-induced phosphorylation of c-Jun and expression of c-Fos. Treatment with apigenin and luteolin also inhibited ERK, JNK and p38 activation. These results suggested that apigenin and luteolin can inhibit UVA-induced MMP-1 and -13 expressions might be mediated through the AP-1 inactivation and inhibition of ROS formation in HaCaT cells. In conclusion, the results presented that apigenin and luteolin may be useful in the prevention of UVA-induced photoaging.