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자외선에 의해 유도되는 Matrix Metalloproteinase 활성 및 발현에 대한 apigenin과 luteolin의 저해 효과

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Author(s)
오교녀
Issued Date
2006
Abstract
Exposure of ultraviolet (UV) light for long term and repeated on the skin often induces chronic skin diseases such as skin cancer as well as photoaging, and the mechanisms of these skin damages are closely associated with up-regulation of collagenases activities. Moreover, UV-induced collagenases cause connective tissue damage and the skin to become wrinkled and aged. This study investigated the effect of apigenin and luteolin on the ultraviolet A (UVA)-induced matrix metalloproteinases (MMP-1 (collagenase-1) and MMP-13 (collagenase-3)) expression in HaCaT human keratinocytes. Treatment with apigenin and luteolin decreased the UVA-induced generation of reactive oxygen species (ROS). In addition, apigenin and luteolin significantly suppressed the UVA-induced expression of MMP-1 and -13 mRNA. Moreover, transient transfection assay using activator protein-1 (AP-1)-linked luciferase reporter gene assay revealed that apigenin and luteolin reduced UVA-induced AP-1 transcriptional activity. In this study investigated the effects of apigenin and luteolin on UVA-induced c-Jun and c-Fos expression in HaCaT cells. Apigenin and luteolin inhibited UVA-induced phosphorylation of c-Jun and expression of c-Fos. Treatment with apigenin and luteolin also inhibited ERK, JNK and p38 activation. These results suggested that apigenin and luteolin can inhibit UVA-induced MMP-1 and -13 expressions might be mediated through the AP-1 inactivation and inhibition of ROS formation in HaCaT cells. In conclusion, the results presented that apigenin and luteolin may be useful in the prevention of UVA-induced photoaging.
Alternative Title
Protective effects of apigenin and luteolin on ultraviolet A-induced Matrix Metalloproteinase expression in human keratinocyts
Alternative Author(s)
Oh, Kyo Nyeo
Affiliation
조선대학교 대학원
Department
일반대학원 약학과
Advisor
鄭惠光
Awarded Date
2007-02
Table Of Contents
LIST OF FIGURES = ⅲ
LIST OF ABBREVIATIONS = ⅳ
ABSTRACT = ⅴ
Ⅰ. 서론 = 1
Ⅱ. 재료 및 방법 = 6
Ⅱ-1. 재료 및 시약 = 6
Ⅱ-2. 세포배양, 시약 처리 및 UVA 조사 = 6
Ⅱ-3. 세포독성 측정 = 7
Ⅱ-4. 활성산소종 (ROS) 생성능 측정 = 7
Ⅱ-5. MMP-1 및 -13 효소활성 측정 = 8
Ⅱ-6. MMP-1 및 -13 유전자 발현 측정 = 8
Ⅱ-7. 형질전환 = 9
Ⅱ-8. c-Jun 및 c-Fos 유전자 발현 측정 = 9
Ⅱ-9. c-Jun 및 c-Fos 단백질 발현 측정 = 10
Ⅱ-10. MAP kinase 활성 측정 = 11
Ⅱ-11. 통계학적 분석 = 12
Ⅲ. 결과 = 15
Ⅲ-1. Apigenin과 luteolin의 세포독성 = 15
Ⅲ-2. 활성산소종 (ROS) 형성에 대한 영향 = 17
Ⅲ-3. MMP-1 및 -13 효소 활성 저해 = 19
Ⅲ-4. MMP-1 및 -13 유전자 발현 억제 = 22
Ⅲ-5. AP-1 전사인자 활성도 = 24
Ⅲ-6. c-Jun 및 c-Fos 유전자 발현 = 26
Ⅲ-7. c-Jun 및 c-Fos 단백질 발현 = 28
Ⅲ-8. MAP kinases 활성 = 30
Ⅳ. 고찰 = 33
Ⅴ. 참고문헌 = 36
Ⅵ. 감사의 글 = 42
Degree
Master
Publisher
조선대학교 대학원
Citation
오교녀. (2006). 자외선에 의해 유도되는 Matrix Metalloproteinase 활성 및 발현에 대한 apigenin과 luteolin의 저해 효과.
Type
Dissertation
URI
https://oak.chosun.ac.kr/handle/2020.oak/6699
http://chosun.dcollection.net/common/orgView/200000234151
Appears in Collections:
General Graduate School > 3. Theses(Master)
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