생쥐 상아모세포 분화과정에서 분비백형구단백분해효소 억제제(SLPI)의 발현

Abstract

The biological function of secretory leukocyte protease inhibitor (SLPI)is believed to be the inhibition of elastase, cathepsin G and other protease, thereby protecting tissue from self-degradation by these enzyme. Tooth development is associated with morphological and biochemical changes of the dental papilla and enamel organ. Among the proteins involved in the development, some proteases and inhibitors have important role for reorganization of the each developing cells. The SLPI is thought as a regulating protein on the synthesis and degradation of matrix proteins. But there was no report of expression of this molecule on the tooth development, especially on the odontoblast. Therefore, the aim of this study is to verify the role and expression of the SLPI during odontogenesis and mineralization during mouse tooth development. From in-situ and immunohistochemical analysis, it has firstly described that the SLPI is expressed in odontoblasts during tooth development. Expression of the SLPI was increased in differentiated odontoblast than that of young odontoblast and at the same time, collagen was also observed. Expression of the SLPI and type I collagen was also expressed during mineralization using MDPC-23 cells. From these results, the expression of SLPI in odontoblast may prevent the degradation of collagen through the regulation of MMP, suggesting that the SLPI may play the important roles in odontogenesis and mineralization.