배양망막색소상피에서 혈관형성관련인자에 대한 트리암시놀론의 효과

Abstract

Purpose : To elucidate triamcinolone acetonide(TA) effect on angiogenesis-related factors of cultured retinal pigment epithelial cells(ARPE-19 cells). Methods : ARPE-19 cells were cultured for 24 hours in the hypoxic chamber and then the cells exposed to TA for 6 hours under normal culture condition. Expression and production of angiogenesis-related factors including angiogenic factor, vascular endothelial growth factor (VEGF) and anti-angiogenic factor, pigment epithelium-derived factor (PEDF) were checked by RT-PCR and Western blot, respectively. The induction of the neoangiogenesis by the culture supernatant harvested after exposure to TA was monitored by both tube formation in ECV 304 cell and migration assay of human dermal microvascular endothelial cells. Results : The competitive RT-PCR showed that the expression of VEGF gene in ARPE-19 cells exposed to TA decreased compared to control(p<0.01) but the expression of PEDF gene was not changed. These results corresponded to that of Western blot. Moreover, the tube formation and the migration assay displayed that the angiogenesis were decreased under the exposure to TA. Conclusions : These results suggest that TA have anti-angiogenic effect with its effect of down-regulation to VEGF in ARPE-19 cells.