방사선조사 마우스에서 알긴산올리고당의 방사선 방어효과에 관한 생화학적 연구

Abstract

In order to find out the Radioprotective effect of algin-oligosaccharide(AOS), this study, with a mouse of which whole frame irradiated by 3Gy radiation once, first, measured caspase-3 and caspase-9 amid cell signaling connected to apoptosis in order to observe cell activation, second, measured NO(nitric oxide) to observe cell inflammation, and third, measured TNF(tumor necrosis factor)-α and IL(interleukin)-6 in order to look at the radiation-induced cytokine. In addition, as a part of acquiring supplementary materials related to radioprotective effect of algin-oligosaccharide, it conducted a biochemical analysis for the serum of the same mouse. Each finding was indicated by average and standard deviation (Mean ± S.D.), the significance between each experiment groups was verified by ANOVA-test through the statistical program, SPSS 10.1, and Tukey-test was conducted as post-hoc analysis for multiple comparisons between each experiment groups. Statistical verification was treated by significant level, α = 0.05. In Caspase-3 and Caspase-9 test for observing cell activation, both of Caspase-3 and Caspase-9 showed highly increased O.D. value in the irradiation control group, while the whole groups treated with algin-oligosaccharide before or after irradiation indicated lower O.D. value than the irradiation control group, especially showed big difference in 7 day's treatment group of before irradiation (P<0.001). It confirmed that Caspase generation was restrained in AOS treatment group. In Nitric Oxide test for observing cell inflammation reaction, small intestine and liver tissue generate less Nitric Oxide in the irradiation control group, while 3 day's treatment group of before irradiation showed significant increase of generation (P<0.001), which confirmed algin-oligosaccharide increased creation of Nitric Oxide. In TNF-α test amid radiation-induced cytokine, small intestine and liver tissue indicated highly increased TNF-α value in the irradiation control group, while small intestine tissue showed restrained generation of TNF-α in 7 day's treatment group of before irradiation (P<0.001), and liver tissue showed suppressed creation of TNF-α in 7 day's treatment group of before irradiation, 3 day's treatment group of before irradiation, and 7 day's treatment group of after irradiation (P<0.001). The finding confirmed TNF-α generation was restrained in AOS treatment group. In IL-6 test amid radiation-induced cytokine, small intestine and liver tissue indicated highly increased IL-6 value in the irradiation control group, while restrained generation of IL-6 was shown in AOS treatment group (p<0.001), which confirmed IL-6 generation was restrained in AOS treatment group. Consequently, this study inquired into the fact that algin-oligosaccharide with superior antioxidant activity performed radiation protection by inducing restraint of Caspase generation, increase of Nitric Oxide generation, restraint of TNF-α and IL-6 generation, and confirmed that natural product with less chemical toxicity was able to be applied as radioprotector.