망막색소상피에서 생산된 VEGF에 대한 Bevacizumab의 영향

Abstract

Purpose: To elucidate the mechanism of anti-Vascular endothelial growth factor (VEGF) effect of bevacizumab (Avastin) at the cultured media of human retinal pigment epithelium (ARPE-19 cells). Methods: ARPE-19 cells were cultured for 24 hours in the hypoxic chamber and then media was treated with Avastin for an hour. Production of VEGF was checked by Western blot. The induction of the neoangiogenesis by the culture supernatant harvested after exposure to hypoxic status and Avastin was monitored by both tube formation in ECV 304 cell and migration assay of human dermal microvascular endothelial cells. Results: Western blot showed that the production of VEGF protein in ARPE-19 cells was decreased by a dose dependent manner after exposure to Avastin (p<0.05). With Avastin treatment on culture media, tube-like structures of ECV-304 cells are decreased (p<0.05). The degree of migration activity of ECV-304 cells decreased according to Avastin concentration (p<0.05). Conclusions: These results suggest that Avastin inhibit the neoangiogenesis at age-related macular degeneration by the decrease of VEGF activity on the cell migration and tube formation.