골아세포가 배양된 치과 임플란트용 Ti-Ta합금의 전기화학적 특성

Abstract

Electrochemical characteristics of osteoblast cell covered Ti-Ta alloy with low elastic modulus were studied. In this study, electrochemical behaviors of surface modified and MC3T3-E1 cell cultured Ti-30Ta alloys have been investigated using various electrochemical methods. The Ti alloys containing Ta were melted by using a vacuum furnace and then homogenized for 6 hrs at 1000℃. MC3T3-E1 cell culture was performed with MC3T3-E1 mouse osteoblasts for 2 days. The microstructures were conducted by X-ray diffraction meter (XRD) and field emission scanning electron microscope (FE-SEM). The corrosion resistance was measured using Electrochemical impedance spectroscopy (1000 ㎑ ~ 10 ㎒: M-1025, Potentiostat, Model 263, EG&G, USA) and Potentiodynamic (- 1500 ㎷ ~ + 2000 ㎷) test in artificial saliva solution at 36.5 ± 1℃. The results were as follows.: 1. From the microstructure analysis, Ti-Ta showed the martensite structure of α + β phase. The micro-structure was changed from lamellar structure to needle-like structure as Ta content increased. 2. Morphology of MC3T3-E1 cell cultured on the Ti-Ta alloy have a quite round form. 3. From corrosion test of MC3TC-E1 cell cultured Ti-Ta alloy, corrosion resistance increased as Ta content increased. Corrosion resistance of cell cultured Ti-Ta alloy increased predominantly in compared with non cell cultured Ti-Ta alloy due to inhibition of the dissolution of metal ion by covered cell. 4. From the analysis AC impedance analysis in artificial saliva solution, Rp value of MC3T3-E1 cell cultured Ti-40 Ta alloy showed 1.60×106 Ωcm2 which was higher than those of other Ti alloy. Polarization resistance of cell-cultured Ti-Ta alloy increased in compared with non-cell cultured Ti alloy. In conclusion, corrosion resistance of MC3T3-E1 cell cultured Ti-Ta alloy increased in compared with non-cell cultured Ti-Ta alloy.