간헐적 교정력 적용 후 백서 치주인대에서 UNC-50의 발현

Abstract

Objective: Periodontal ligament fibroblasts have an ectomesenchymal origin and are thought to play crucial roles for not only homeostasis of periodontal tissue but also bone remodeling, wound healing and regeneration of tissue. Recently, it has been reported that UNC-50 is not expressed in gingival fibroblast but in PDL fibroblast. The purpose of this study was to examine the expression of UNC-50 and osteoclacin in periodontium of after application of intermittent force. Methods: Twelve rats had 40 grams of mesially directed force applied at the upper molar for 1 hour/day. Four rats were sacrificed at 1, 3, and 5 days. Immunohistochemical localization of UNC-50 and osteocalcin antibody was carried out. The results showed apposition of new cellular cementum and a slight increase in periodontal space at the tension side. Results: Strong UNC-50 expression was observed in the differentiating cementoblasts close to PDL fibroblasts in tension side whereas it was barely expression in compression side. Expression was strong at day 3, and decreased at day 5. Osteoclacin immunoreactivity expression was strong in differentiating cementoblasts at the tension side. Conclusion: It can be suggested that UNC-50 is related to the differentiation of cementoblasts, and may be responsible for the molecular event in PDL cells under mechanical stress.