Tetracycline-HCl이 사람치주인대세포의 MMP-1, MMP-8, MMP-13, 및 TIMP-1의 발현에 끼치는 효과

Abstract

The integrity of gingival connective tissues surrounding tooth may be influenced by a balance between maxtrix metalloproteinases (MMPs) and tissue inhibitor of matrix metalloproteinse (TIMP). The aim of this study was to analyse the level of MMPs such as MMP-1, MMP-8, and MMP-13 and TIMP-1 expressed by human periodontal ligament fibroblasts (hPDLF) via western blotting method. hPDLFs were obtained from a healthy periodontium and cultured in Dulbecco's modified Eagles's medium plus 10% fetal bone serum. The concentration of tertacycline-HCl (TC-HCl) application in hPDLF was 250 ㎎/㎖, 250 ㎎/5㎖ 250 ㎎/10㎖ and 250 ㎎/100㎖. TC-HCl was applied with hPDLFs for 1-min. a day over 5 days, respectively. The gene expression on the proenzyme forms and/or enzyme forms of MMP-1-, MMP-8-, MMP-1-TIMP-13-, and TIMP-1-mRNA in hPDLFd was analysed at 6-day after TC-HCl application via western blotting method. Within the limited experiments, author confirmed that TC-HCl application decreased mRNA induction for MMPs tested, except enzyme form of 64kD of MMP-8 and increased for TIMP, in hPDLF, In case of negative control, active forms of MMPs (42kD MMP-1, 22kd MMP-8, 48 kd MMP-13) increased were more expressed than inactive form of MMPs. In future, more research will be needed in order to evaluate the roles of different active enzyme forms of MMPs according to TC-HCl application in hPDLF from a experimental standpoint.