Rosmarinic acid의 생합성 및 효과에 관한 연구 : 배초향 모상근을 통한 rosmarinic acid 생산과 항산화, 항암효과

Abstract

Agastache rugosa Kuntze belonging to Korean mint family (Labiatae) is a perennial herb and is widely distributed in the East Asian countries with important role in the traditional medicine of several Oriental cultures. One of the main active constituents of Korean mint is rosmarinic acid which contains attributes like antioxidant and allelochemical properties. Agrobacterium rhizogenes is a genus of gram-negative soil bacteria belonging to the Rhizobiaceae. A. rhizogenes can transfer T-DNA, excised from Ri(root inducing)-plasmids several hundred kb in size on the bacterial in the plant cell. It is the causal agent of 'hairy root' diseases in plants and used for the production of hairy root cultures from a multitude of species. Hairy root cultures of Agastache rugosa K. were established by infecting leaf explants with Agrobacterium rhizogenes R1000. Hairy roots were cultured in MS liquid medium and the maximum growth (219mg dry wt/ flask) was already reached by 30 days of culture with content of rosmarinic acid (0.14g/g dry wt). This result demonstrates that Korean mint hairy culture can be a valuable alternative approach for the production of rosmarinic acid. Hairy root cultures from plants have attracted considerable attention because of their genetic and biochemical stability, rapid growth rate and ability to synthesize secondary products at levels comparable to the original plants.