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연근의 생리활성 및 이용실태에 관한 연구

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Author(s)
박세영
Issued Date
2006
Abstract
The node of lotus rhizome(Nelumbo nucifera G. root) has been used for the remedy of bleeding, blood stagnancy, thirstiness, anti-diabetic and antipyretic activity as a traditional medicine in Korea. In this study, the effects of ethanol extract of lotus root(LRE) on antioxidant capacity and lipid metabolism were investigated. In addition, usage of lotus root at food service, such as school, industry and hospital, in Jeollanamdo-Gwangju areas was also surveyed to provide basic data for dietitians.
This study investigated the effects of an ethanol extract of lotus root on alcohol-induced hepatotoxicity in the rat. Sprague-Dawley rats weighing 100~150g, were divided into 6 groups: basal diet group(BD), alcohol(35%, 10 mL/kg/day) treated group(ET), LRE 200 mg/kg/day treated group(BD-LREL), LRE 400 mg/kg/day treated group(BD-LREH), LRE 200 mg/kg/day and alcohol treated group(ET-LREL), and LRE 400 mg/kg/day and alcohol treated group(ET-LREH). Lotus root ethanol extract was fractionated by the following: n-hexane, chloroform, ethylacetate and n-butanol. The antioxidative capacity of the ethylacetate fraction was the highest among fractions. The body weight gain was decreased by alcohol administration, however, was gradually increased to a little lower level than the basal diet group by administering lotus root ethanol extract. The serum alanine aminotransferase(ALT), asparate aminotransferase(AST) and alkaline phosphatase(ALP) activities that were elevated by alcohol were significantly decreased by lotus root ethanol extract administration. These effects of lotus root ethanol extract within the alcohol groups were dose-dependent manners. It was also observed that thiobarbituric acid reactive substances(TBARS) content, xanthine oxidase(XO), superoxide dismutase(SOD), catalase and glutathione peroxidase(GSH-Px) activities in liver that were increased by alcohol were markedly decreased in the lotus root ethanol extract administered groups as compared with the alcohol administrated group. These effects of lotus root ethanol extract within the alcohol groups were dose-dependent manners. The glutathione(GSH) concentration in liver was decreased by alcohol adminstration, however, increased after administering lotus root ethanol extract.
Next, this study was also performed to investigate the effects of lotus root ethanol extract on serum and liver lipid metabolism in rats. Lotus root ethanol extract(200 mg/kg/day and 400 mg/kg/day) was administered orally to rats with fed high cholesterol diet for 6 weeks. We divided into 6 groups: normal diet group(NC), high cholesterol diet group(1% cholesterol and 0.25% sodium cholate)(HC), LRE 200 mg/kg treated group(NC-LREL), LRE 400 mg/kg treated group(NC-LREH), high cholesterol diet and LRE 200 mg/kg treated group(HC-LREL), and high cholesterol diet and LRE 400 mg/kg treated group(HC-LREH). The inhibitory effects of lotus root ethanol extract on LDL-cholesterol oxidation and HMG-CoA reductase activity of in vitro were observed. The growth rate and liver weight of the high cholesterol diet group was higher than the normal diet group, whereas the groups administered lotus root ethanol extract were gradually decreased. There was a signigicant increase in the activities of serum ALT, AST and ALP in the high cholesterol diet group. The administration of lotus root ethanol extract decreased serum ALT, AST and ALP activities in dose dependent manners. The high cholesterol diet group was increased serum triglyceride, total cholesterol, free cholesterol and LDL-cholesterol levels, and decreased atherogenic index, HDL-cholesterol and phospholipid levels as compared with the normal diet group. Lotus root ethanol extract administrated groups were increased HDL-C/T-C, HDL-cholesterol and phospholipid levels, and decreased serum triglyceride, total cholesterol, free cholesterol, and LDL-cholesterol levels as compared with the high cholesterol diet group. The hepatic concentrations of total lipid, total cholesterol and triglyceride were also lower in lotus root ethanol extract administrated groups than in the high cholesterol diet group.
We also surveyed dietitians' perception on usuage of lotus root. This study was carried out through a questionnaire and the subjects was 131 school dietitans, 72 industry dietitans and 45 hospital dietitans in Jeollanamdo-Gwangju areas. According to the survey, most dietitians perceived that minimally processed ready-to-use lotus root would helpful for the institutional food service. Most respondents applied lotus root to their menu planning and used lotus root for food service meal less than 10 times per years. Lotus root jorim was estimated the most preferred cooking method. Major reasons for their using lotus root were health food and characteristic food, and reasons for non-use of lotus root were simple cooking method, high price and less preference. It is needed to study for the development of cooking method of lotus root proper to preference of student, adolescent and patient in food service. In addition, for patients' nutrition and well-being through institutional food service meal, it is necessary to prepare database of vegetables such as lotus root.
Taken together, these results suggest that ethanol extract of lotus root may have a possible protective effect on liver function in hepatotoxicity-induced rat by alcohol administration, fatty liver in hyperlipidemia-induced rat by a high cholesterol diet. The development of cooking recipe with lotus root and their antioxidative and hyperlipidermic effects of lotus root on patients need to be investigated further.
Alternative Title
A Study on the Biological Activity and Consumption of Lotus Root(Nelumbo nucifera Gaertn)
Alternative Author(s)
Park, Se-Young
Affiliation
조선대학교 대학원
Department
일반대학원 식품영양학과
Advisor
李明烈
Awarded Date
2006-08
Table Of Contents
ABSTRACT = xiv
제1장 서론 = 1
제2장 재료 및 방법 = 8
제1절 실험재료 = 8
제2절 시약 및 실험기기 = 8
1. 시약 = 8
2. 실험기기 = 9
제3절 시료추출 = 9
1. 연근 에탄올 추출 = 9
2. 에탄올 추출물의 분획 = 10
제4절 항산화 활성 실험 = 12
1. In vitro 에서 항산화 활성 측정 = 12
가. 1.1-diphenyl-2- picrylhydrazyl(DPPH) radical 소거능 = 12
나. 항산화지수 = 12
다. 아질산염 소거능 = 13
라. 지질과산화 억제 효능 = 13
2. In vivo 에서 항산화 효능 실험 = 14
가. 실험동물 사육 = 14
나. 실험설계 및 실험식이 = 15
다. 실험동물 처치 = 15
라. 혈청 중 AST, ALT 및 ALP 활성 측정 = 19
마. 간조직 중 효소 활성 측정 = 19
(1) 효소원 조제 = 19
(2) Xanthine oxidase(XO) 활성 = 19
(3) Superoxide dismutase(SOD) 활성 = 20
(4) Catalase 활성 = 20
(5) Glutathione peroxidase(GSH-Px) 활성 = 21
(6) Glutathione(GSH) 함량 = 21
(7) Thiobarbituric acid reactive substances(TBARS) 함량 = 22
(8) 단백질 함량 = 22
바. 통계처리 = 22
제5절 고지혈증 억제 효능 실험 = 24
1. In vitro 에서 고지혈증 억제효과 측정 = 24
가. Low density lipoprotein(LDL)에 대한 항산화효과 측정 = 24
(1) LDL 분리 = 24
(2) LDL 산화 및 항산화효과 측정 = 24
나. 연근의 HMG-CoA reductase 저해활성 측정 = 25
(1) Yeast 배양 = 25
(2) Microsomal protein 제조 = 25
(3) HMG-CoA reductase 저해활성 = 25
2. In vivo 에서 고지혈증 억제 효능 실험 = 27
가. 실험동물 사육 = 27
나. 실험설계 및 실험식이 = 27
다. 실험동물의 처치 = 27
라. 혈청 중 ALP, AST 및 ALP 활성 측정 = 30
마. 혈청 중 지질 함량 측정 = 30
바. 간조직 중 지질 함량 측정 = 30
사. 통계처리 = 31
제6절 단체급식소의 연근 이용실태 조사 = 32
1. 연구대상 및 기간 = 32
2. 연구방법 및 내용 = 32
3. 통계분석 = 32
제3장 실험결과 및 고찰 = 34
제1절 항산화 효능 = 34
1. 80% 에탄올 추출물 수율 및 용매별 분획물 수율 = 34
2. In vitro 에서 항산화 활성 = 36
가. DPPH radical 소거작용 = 36
나. 항산화지수 = 38
다. 아질산염 소거능 = 40
라. 지질과산화 억제 효능 = 42
3. In vivo 에서 항산화 효능 = 44
가. 체중증가율 및 식이효율 = 44
나. 혈청 중 ALT, AST 및 ALP 활성 = 47
다. 간조직 중 항산화 효소 활성 = 51
(1) XO 활성 = 51
(2) SOD, catalase 및 GSH-Px 활성 = 53
라. 간조직 중 GSH 함량 = 59
마. 간조직 중 지질과산화물 함량 = 62
제2절 고지혈증 억제 효능 = 64
1. In vitro 에서 고지혈증 억제 효능 = 64
가. 산화된 LDL에 대한 항산화 효능 = 64
나. HMG-CoA reductase 저해활성 = 66
2. In vivo 에서 고지혈증 억제 효능 = 69
가. 체중증가율, 식이효율 및 간 무게 = 69
나. 혈청 중 ALT, AST 및 ALP 활성 = 74
다. 혈청 중 중성지방, 총콜레스테롤 및 인지질 함량 = 79
라. 혈청 중 유리 콜레스테롤 함량, 콜레스테릴 에스테르 함량 및 콜레스테릴 에스테르 비율 = 84
마. 혈청 LDL-콜레스테롤 함량, HDL-콜레스테롤 함량, 심혈관 위험지수 및 동맥경화지수 = 89
바. 간조직 중 총지질, 총콜레스테롤 및 중성지방 함량 = 96
제3절 단체급식소의 연근이용 실태 = 101
1. 조사대상자의 일반적인 사항 = 101
가. 조사대상 영양사의 일반적 특징 = 101
나. 조사급식소의 급식유형별 운영 현황 = 104
2. 연근의 이용실태 = 107
가. 연근 사용빈도 = 107
나. 연근 구입할 때 상태 = 110
다. 연근 1회 사용 분량 = 112
라. 연근 조리법의 이용 실태 = 115
마. 연근의 사용에 대한 영양사의 인식조사 = 118
바. 연근 조리의 잔반비율 = 121
제4장 요약 = 123
제5장 참고문헌 = 128
설문지 = 147
Degree
Doctor
Publisher
조선대학교 대학원
Citation
박세영. (2006). 연근의 생리활성 및 이용실태에 관한 연구.
Type
Dissertation
URI
https://oak.chosun.ac.kr/handle/2020.oak/6392
http://chosun.dcollection.net/common/orgView/200000233034
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General Graduate School > 4. Theses(Ph.D)
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