수종의 치주질환원인균에 대한 Propolis의 항균효과

Abstract

The aim of this study was to investigate the antimicrobial effect of Propolis on periodontopathogens. To test the antimicrobial effect of Propolis the minimum bactericidal concentration (MBC) of against 2 strains of Actinobacillus actinomycetemcomitans, 1 strains of Fusobacterium nucleatum, 1 strains of Prevotella intermedia, 2 strains of Prevotella nigrescens, 1 strains of Porphyromonas gingivalis, and 1 strains of Tannerella forsythia was measured by broth dilution method. Time-kill curves were assessed for susceptible bacteria, testing 0, 0.5, 1, 2 × MBC for Propolis, by counting viable bacteria after 3, 90, 180, 360, 720, 1440 minutes. The minimum bactericidal concentration of Propolis was 4.8, 9.3, 9.3, 9.3, 18.9, 37.5 and 75 ?g/ml on the F. nucleatum ATCC 51190, P. gingivalis ATCC 33277, P. intermedia ATCC 49046, P. nigrescens ATCC 33563, A. actinomycetemcomitans ATCC 43718, P. nigrescens ATCC 25261, A. actinomycetemcomitans ATCC 33385 respectively. Time-kill curves demonstrated Propolis had bactericidal activity on all strains. The results suggest that Propolis can be useful in the agents for the prevention and treatment of periodontitis. Key words: antimicrobial effect, Propolis, Periodontopathogens