수용성β-1,3-glucan 생산을 위한 β-1,3-glucan synthase 클로닝 및 재조합 대장균 개발

Abstract

The water-soluble β-1,3-glucan is produced by recombinant E.coli in fermentation reactor. Following β-1,3-glucan synthesis metabolic pathway, UDP-glucose is the exclusive precursor for the synthesis of β-1,3-glucan. But UDP-glucose on as much as β-1,3-glucan production outsides the conversion is possible even with the UDP-galactose. When the UDP-glucose increases conversions with β-1,3-glucan, it is that β-1,3-glucan synthase do to be revelation plentifully. In this study, for increase UDP-glucose to β-1,3-glucan reaction, we developed recombinant E.coli contain β-1,3-glucan synthase gene site. For a develop recombinant E.coli , From the Data Base of the NCBI the Putative of Agrobacterium sp.ATCC31749 β-1,3-glucan synthase catalytic subunit (gi:40556679) it used gene site. Finally, the PCR reaction it amplified the DNA fragment and using electrophoresis confirmed DNA fragment size. That purified protein have mass of 72kDa, 62KDa, 42KDa, 31KDa on SDS-PAGE respectively.