모과 에탄올 추출물이 흰쥐의 항산화계에 미치는 영향

Abstract

This study was performed to investigate the effect of ethanol extract of Chaenomeles sinensis Koehne(CS) on antioxidant activities system in rats. The results obtained were as follows : 1. Physicochemical components of dried CS were investigated. CS contained 20.57% of moisture, 16.06% of crude protein, 1.40% of crude lipid, 11.03% of ash, and 50.94% of carbohydrate. The contents of vitamin A and vitamin E were 0.040 mg/100g, and 0.275 mg/100g, respectively. Predominant minerals were K, Ca, Mg and Fe. The results of amino acid analysis showed that glutamic acid, aspartic acid, lysine, leucine, valine and alanine were the most abundant amino acids in sample. Organic acids including malic acid, citric acid, oxalic acid, succinic acid and formic acid were detected. The major fatty acids were palmitic acid and linoleic acid. 2. This study was also investigated on the antioxidative activity of 80% ethanol extracts from CS. The extraction yields of 80% ethanol extract was 29.33 g/100g. The extract was further fractionated subsequently by n-hexane, chloroform, ethylacetate, butanol and water. n-hexane fraction showed the highest total flavonoid and polyphenol contents. In vitro, antioxidative activities were examined by DPPH, Rancimat test, and thiobarbituric acid(TBA) value, in comparison with commercial antioxidant. Antioxidant index of the n-hexane fraction of CS ethanol extract was the highest among fractions. 3.The effects of an ethanol extract of CS on the ethanol-induced hepatotoxicity in rat liver were also investigated. Sprague-Dawley rats weighing 135～155g, were divided into 6 groups; normal group(ND), alcohol(35%, 10 mL/kg) treated group(ET), CS ethanol extract 200 mg/kg treated group(ND-CSL), CS ethanol extract 400 mg/kg treated group(ND-CSH), CS ethanol extract 200 mg/kg and alcohol treated group(ET-CSL), and CS ethanol extract 400 mg/kg and alcohol treated group(ET-CSH). ① There were no differences between the ND group and the ET group in the body weight gain and feed effeciency. ② There were increases in the activities of serum alanine aminotransferase(ALT), asparate aminotransferase(AST), and alkaline phosphatase(ALP) in the ET group. On the other hand, the administration of CS decreased ALT, AST and ALP activities in serum. These effects of CS within ET groups were dose-dependent. ③ It was also observed that the hepatic activities of superoxide dismutase(SOD), catalase, GSH-Px and xanthine oxidase(XO) that were increased by alcohol were also markedly decreased in the CS administered groups as compared with the ET group. These effects of CS within ET groups were dose-dependent. There were no differences in the activities of liver SOD, catalase, GSH-Px and XO between normal diet groups(ND, ET, ND-CSL, ND-CSH, ET-CSL, ET-CSH) The TBARS contents were increased by the ET group, on the other hand, the administration of CS reduced TBARS value in liver. In addition, the content of glutathione(GSH) in liver was decreased by alcohol administration, however, increased after administering CS. In conclusion, the administration of alcohol develops the hyperoxidation of liver lipid through the increase of activity of enzyme related to the lipid peroxiation, however, decreased after administring CS. Thus, CS may have a protective effect on ethanol-induced hepatotoxicity in rat liver.