치과용 Ti합금의 내식성에 미치는 표면거칠기와 세포배양효과

Abstract

Effects of surface roughness and cell culture on the corrosion resistance of Ti alloy for dental use was studied. This study investigated the corrosion resistance and surface roughness of surface modified and MC3T3 E-1 cell cultured Ti-6Al-4V substrates. Samples with four different surface preparations were used: 0.3 ㎛ polished, 25 ㎛, 50 ㎛ and 125 ㎛ sandblasted. Cell culture was performed with MC3T3 E-1 mouse osteoblasts for 2 days. Average surface roughness and surface morphology was observed using roughness tester(DSF-1000, kosaka, Japan). The corrosion resistance was measured using Electrochemical impedance spectroscopy(1000㎑ ～ 1㎒: M-1025, Potentiostat, Model 263, EG&G, USA)) and Potentiodynamic(-1000 ㎷ ～ 1600 ㎷) test in the artificial saliva solution. The results were as follows.: 1. The surface roughness of polished Ti alloy were smoother than that of 25, 50 and 125 ㎛ sandblasted Ti alloy. 2. Morphology of MC3T3 E-1 cell cultured on the Ti-6Al-4V alloy have a quite round form. In the case of polished sample, Cell was cultured well on the surface of Ti-6Al-4V alloy. Growth and size of cell increased and cytoplasmic prolongations observed at rough surface. 3. From corrosion test of sandblasted and cell cultured Ti-6Al-4V alloy, corrosion resistance decreased as surface roughness increased. Corrosion resistance of cell cultured Ti-6Al-4V alloy increased predominantly in compared with non cell cultured Ti-6Al-4V alloy due to inhibition of the dissolution of metal ion by covered cell.