장기간 알코올 투여에 의한 흰쥐 소뇌에서의 BDNF와 TrkB발현의 감소

Abstract

Brain-derived neurotrophic factor (BDNF) and its specific receptor TrkB are concerned with survival and maintenance of neurons in the mature cerebellum. This study examined the effects of chronic ethanol treatment (CET) on the expression of BDNF and TrkB proteins in the rat cerebellum. CET was induced via daily liquid alcohol intake for 28 weeks beginning at 10 weeks of age. Adult Sprague-Dawley rats (n=24) were divided into two groups according to sex. Immunohistochemistry was performed to detect the localization of BDNF and TrkB proteins, and the number of BDNF- and TrkB-immunoreactive (IR) Purkinje cell was measured in the lobule 2/3 and 4/5 of cerebellum. As a result, BDNF and TrkB immunoreactivities were detected in cell bodies and dendrites of Purkinje cells both in the controls and the CET groups. However, in the molecular layer, the staining intensity and the complexity of dendrite of Purkinje cell were reduced in the CET groups compared to the controls both in male and female. The statistical result also had shown that the CET produced significant reduction of the number of BDNF- and TrkB-IR Purkinje cells both in male and female. On the other hand, there was no sex differences except for BDNF-IR Purkinje cells in the CET group. These results may indicate that CET produces a significant reduction of BDNF and its receptor TrkB expression in the rat cerebellar Purkinje cell.