자색고구마 (Ipomoea batatas L.) 유래 습관성캘러스로부터 안토시아닌 생합성

Abstract

The tissue culture of leaf disks of purple sweet potato (Ipomoea batatas L.) was studied to establish the optimal culture conditions for anthocyanin accumulation and selection of the cell line producing the anthocyanin. The high amount of anthocyanin producing cell line was selected from the suspension culture of habituated calli, which were grown fast on the hormone free Murashige and Skoog basal medium (MS medium). The high pigmented and habituated callus appeared in the suspension culture after 20 weeks subculturing in the MS medium, in which the ability of the cell line to produce a high amount of anthocyanin was maintained. The color value of the pigments extracted from the calli cultured in the MS medium was 1.1 mg/mL which was close to 1.3 mg/ mL that is the value of the pigment extracted from the storage roots of sweet potato. In a suspension culture of the habituated calli, the highest content of anthocyanin and the maximum rate of cell growth were obtained with addition of the 3% sucrose in the MS medium. The most significant enhancement in anthocyanin production was obtained when the jasmonic acid was added to the MS medium. The highest anthocyanin content was 1.2 mg/mL obtained at 12 days after 5 uM jasmonic acid was added. This represented an 3-fold increase compared with the control culture. The maximum weight of the callus was 1.684 g measured at 30 days after 5 uM ABA was added to the MS medium. This value was 1.5-fold of that of the control. According to HPLC analysis, peonidin as a component of anthocyanin was isolated from the highly pigmented and habituated callus.