이질풀(Geranium thunbergii)의 化學成分 및 生理活性에 관한 硏究

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Geranium thunbergii Sieb. et Zucc. (Geraniaceae) is a perennial plantand isdistributed in Korea,China, and Japan. In traditional medicine, the whole plants are used as an antihemorrhage, sterilization, diarrhea, andastringent. Previous phytochemical studies on this species afforded tannins and flavonoids, such as geraniin, corilagin, ellagic acid, gallic acid, quercetin, kaempferol, and kaempferol-7-rhamnoside. Inanongoing investigation into biologically active compounds from natural products, the methylene chloride soluble fraction of methanol extract from Geranium thunbergii Sieb. et Zucc. (Geraniaceae) showed significant antioxidative activity on the NBT superoxide scavenging assay and inhibitory effecton TNF-α inducedhIL-6production in theMG-63 cell. By means of the repeated column chromatography using silica gel, Sephadex LH-20, LiChroprep RP-18, two sterols 7-oxositosterol (1) and sterol mixture (2), three lignans (+)methyl piperitol (kobusin)(3)7,7`-dihydroxy bursehernin(4), 4-α-hydroxykobusin (5), and one flavonol glycosides, kaempferol-3-O-α-L-rhamnopyra noside (6) were isolated. Their structures of compounds 1~6 were determined by chemical analysis, as well as, 1D- with 2D-NMR sepectroscopy. Among them, 4-α-hydroxykobusin (5) was isolated for the first time in nature, and compounds 2~5 were isolated from this plant for the first time. In vitro cell cytotoxicity was evaluated by SRB method and compounds 2~5 showed no cytotoxic activity. Compound 2 and 4 showed inhibitory effect on TNF-α induced hIL-6 production in the MG-63 cell. Therefore, compounds 2 and 4 may be developed for the anti-inflammatory agents and treatment of disease by overexpression of hIL-6.
Alternative Title
Phytochemical constituents and biological activities of Geranium thunbergii Sieb. et Zucc.
Alternative Author(s)
Liu, Qing-He
조선대학교 대학원
일반대학원 약학과
Awarded Date
Table Of Contents
Contents Ⅰ
ListofSchemes Ⅲ
ListofTables = Ⅲ
ListofFigures = Ⅳ
Abstract = Ⅴ
Ⅰ.緖論 = 1
Ⅱ.實驗 = 4
1.實驗材料 = 4
2.試藥 및 器機 = 4
2-1.試藥 = 4
2-2.器機 = 5
3.化合物의 分離 = 6
3-1.抽出및 分劃 = 6
3-2.CH₂Cl₂分劃으로부터 compound의 分離 = 6
3-2-1.Compound 1의 分離 = 9
3-2-2.Compound 2의 分離 = 10
3-2-3.Compound 3의 分離 = 11
3-2-4.Compound 4의 分離 = 13
3-2-5.Compound 5의 分離 = 14
3-2-6.Compound 6의 分離 = 15
4.生理活性 = 17
4-1.NBT superoxidescavengingassay = 17
4-2.Cytotoxicactivityassay = 17
4-3.MG-63에서 hIL-6의 遊離確認 = 19
Ⅲ.結果및 考察 = 21
1.Compound 1의 構造 = 21
2.Compound 2의 構造 = 21
3.Compound 3의 構造 = 22
4.Compound 4의 構造 = 23
5.Compound 5의 構造 = 23
6.Compound 6의 構造 = 24
7.生理活性 結果 = 27
7-1.NBT superoxide scavenging assay = 27
7-2.Cytotoxic activity assay = 27
7-3.MG-63에서 hIL-6의 遊離確認 = 27
Ⅳ.結論 = 29
Ⅴ.參考文獻 = 31
조선대학교 대학원
류경학. (2006). 이질풀(Geranium thunbergii)의 化學成分 및 生理活性에 관한 硏究.
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