손상된 척수에 이식한 사람탯줄혈액이 뇌줄기에 미치는 공초점 주사현미경적 연구

Abstract

Stem cells are a valuable resource for treatment of the disease. but limited access to stem cells from tissues such as brain restricts their utility. Many approaches have been adopted to restore the function following brain stem injury (BSI) and spinal cord injury (SCI). The use of the human umbilical cord blood (hUCB) - a rich source of nonembryonic or adult stem cells - has recently been reported to ameliorate the behavioral consequences of stroke. Forty rats were divided into 8 groups: (1) SCI 1+hUCB (infused 1 day post injury); (2) SCI 2+hUCB (infused 2 days post injury); (3) SCI 3+hUCB (infused 3 days post injury); (4) SCI 4+hUCB (infused 4 days post injury); (5) SCI 5+hUCB (infusedt 5 days post injury); (6) SCI 6+hUCB (infused 6 days post injury); (7) LO+hUCB (laminectomy + hUCB); and (8) LO (laminectomy only). SCI was produced by compressing the spinal cord for one minute with an aneurysm clip calibrated to a closing pressure of 50 g. We report here that immunohistochemical identification of fluorescent hUCB positive cells in the brain stem after compressed spinal cord using mouse anti-human mitochondria monoclonal antibody (MAB1273). All SCI + hUCB(1~8) groups contained fluorescent hUCB positive cells in the all area of the brain stem. Especially, a large number of fluorescent hUCB positive cells also were observed in the whole area of the brain stem of the experimental 5(SCI 5+hUCB) and 6(SCI 6+hUCB)groups. No hUCB positive cells were found in the brain stem area from non-injured spinal cord of these animals or in the animals receiving only a laminectomy was performed. These results suggest that hUCB are potentially useful as a vector for treating a variety of the central nervous system disorders, and we are sure that continuous stem cell study will give an best opportunity to treat the all uncurable organs disorders in the future.